AI Article Synopsis
- A study comparing 490 serum samples from sheep flocks analyzed BHV-5 antibodies using four different tests: ELISA, serum neutralization test, cell assay, and immunofluorescence assay.
- All tests showed consistent results in identifying positive and negative samples, but varied in the percentage of positive samples detected: ELISA (54.7%), cell assay (65.8%), immunofluorescence assay (46.6%), and serum neutralization test (45.1%).
- ELISA, cell assay, and immunofluorescence assay were found to be simpler and faster to perform than the serum neutralization test.
Article Abstract
A comparison of the analyses of 490 serum samples from various flocks of sheep for the detection of BHV-5 antibodies using ELISA, serum neutralisation test, cell assay and immunofluorescence assay showed nearly identical results regarding the distinction between positive and negative serum samples. The percentage of serum samples with antibodies against BHV-5 antigen differed from test to test: ELISA 268 (54.7%), cell assay 323 (65.8%), immunofluorescence assay 222 (46.6%) and serum neutralisation test 221 (45.1%). With ELISA, cell assay and immunofluorescence assay the experiment can be carried out more simply and rapidly than with serum neutralisation test.
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