A microdialysis system with a removable probe and a fixed scleral entry port is presented. The probe can be inserted several times with minimal trauma, permitting repeated sampling in one animal. The functioning of the setup is illustrated by the measurement of dopamine, dihydroxyphenyl acetic acid, and noradrenaline in the vitreous of healthy rabbits under physiological conditions.
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http://dx.doi.org/10.1007/BF00921960 | DOI Listing |
Front Pharmacol
January 2025
Center for Pharmacometrics and System Pharmacology, College of Pharmacy, University of Florida, Orlando, FL, United States.
Introduction: This study evaluated the relationship between total plasma and free kidney concentrations of amphotericin B (AmB) in healthy and -infected Wistar rats using microdialysis and has the potential to significantly impact future research in this field and promote the development of antifungal drugs. The findings of this study, which show that plasma levels are a good predictor for AmB kidney concentrations and can be used to optimize its dosing regimen, underscore the importance of this research.
Methods: Microdialysis probe recovery rates were determined by dialysis and retrodialysis , as well as by retrodialysis .
Nat Prod Res
January 2025
Engineering Research Center of Modern Preparation Technology of TCM of Ministry of Education, Innovation Research Institute of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Borneolum (BO) is an effectiveness adjuvant in facilitating the transportation of central nervous system drugs to the brain by opening the blood-brain barrier (BBB). Citalopram hydrobromide (CIT-HBr), a widely prescribed serotonin (5-HT) reuptake inhibitor, restricts the efficacy due to the BBB, resulting in slow onset and systemic side effects. Enhancing CIT-HBr's efficacy through BO appears to be a promising approach.
View Article and Find Full Text PDFProc Biol Sci
January 2025
Laboratory for Integrative Neuroscience and Endocrinology, University of Bristol, Bristol BS1 3NY, UK.
Cortisol is released upon activation of the hypothalamic-pituitary-adrenal axis, varies across the day, possesses an underlying diurnal rhythm and is responsive to stressors. The endogenous circadian peak of cortisol occurs in the morning, and increases in cortisol observed post-awakening have been named the cortisol awakening response (CAR) based on the belief that the act of waking up stimulates cortisol secretion. However, objective evidence that awakening induces cortisol secretion is limited.
View Article and Find Full Text PDFJ Neurosci Methods
March 2025
Cognitive Neuroscience Laboratory, German Primate Center - Leibniz Institute for Primate Research, Goettingen, Germany; Faculty of Biology and Psychology, University of Goettingen, Goettingen 37077 Germany.
Background: Neuronal activity is modulated by behavior and cognitive processes. The combination of several neurotransmitter systems, acting directly or indirectly on specific populations of neurons, underlie such modulations. Most studies with non-human primates (NHPs) fail to capture this complexity, partly due to the lack of adequate methods for reliably and simultaneously measuring a broad spectrum of neurotransmitters while the animal engages in behavioral tasks.
View Article and Find Full Text PDFTalanta
December 2024
Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, College of Chemistry and Chemical Engineering, Hubei Normal University, Huangshi 435002, PR China.
In this study, we demonstrate that a highly efficient colorimetric sensor prepared from carbon-shielded Co-Ce Prussian blue analog (PBA) nanopetals (CoO/CeO@C) by green chemical deposition method and thermal annealing processes for detection of ascorbic acid (AA) in cerebral microdialysis fluids. The synthesized CoO/CeO@C showed high dual-mimetic activity, i.e.
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