AI Article Synopsis
- A straightforward and accurate densitometric technique for measuring vanillylmandelic acid (VMA) in urine is proposed.
- The method involves direct urine application, thin-layer chromatography (TLC) separation, derivatization, and measuring the resulting derivative at 560 nm.
- Quantification is achieved using a TLC Scanner and a five-point calibration, with urinary VMA concentrations found to range between 4.84 and 56.06 mumol/day.
Article Abstract
We propose a simple and precise densitometric method for measuring vanillylmandelic acid (VMA). The method comprises direct urine application, thin-layer chromatographic (TLC) separation, postchromatographic derivatization, and in situ reading of the formed derivative at 560 nm. Quantitation was done by measuring peak area with a computer-controlled TLC Scanner and applying a five-point calibration function. The calibration function was 7.57-49.23 mumol/L. This method was used to determine urinary concentrations of excreted VMA; concentrations ranged from 4.84 to 56.06 mumol/day.
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