Conformational changes of lambda phage DNA macromolecules were investigated by dynamic light scattering (DLS) method. It was shown that the persistence length of B-form DNA in some conditions could could decline to approximately 12 nm. When studying the internal motions in the linear form of phage DNA macromolecule, a deviation was observed of the experimental angular dependence of the first cumulant gamma from theoretical predictions for the gamma in Rouse-Zimm model including hydrodynamic interactions. This deviation appeared in the region qRg >> 1, where Rg is gyration radius and q is momentum vector. The first cumulant deviation to higher frequencies is observed only for B-form DNA. Experimental behaviour of the first cumulant gamma for the A-form DNA coincides with the theoretical one in the Rouse-Zimm model.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Isolation, characterization, and genome sequencing analysis of a novel phage HBW-1 of Salmonella.
Microb Pathog
January 2025
Laboratory of Molecular Microbiology and Food Safety, Zhejiang University College of Animal Sciences, Hangzhou 310058, China; Hainan Institute of Zhejiang University, Sanya 572025, China; Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China. Electronic address:
Salmonella presents a significant threat to the health of animals and humans, especially with the rise of strains resistant to multiple drugs. This highlights the necessity for creating sustainable and efficient practical approaches to managing salmonellosis. The most recent and safest approach to combat antimicrobial resistance-associated infections is lytic bacteriophages.
View Article and Find Full Text PDFThe activity of indigo carmine against bacteriophages: an edible antiphage agent.
Appl Microbiol Biotechnol
January 2025
Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224, Warsaw, Poland.
Bacteriophage infections in bacterial cultures pose a significant challenge to industrial bioprocesses, necessitating the development of innovative antiphage solutions. This study explores the antiphage potential of indigo carmine (IC), a common FDA-approved food additive. IC demonstrated selective inactivation of DNA phages (P001, T4, T1, T7, λ) with the EC values ranging from 0.
View Article and Find Full Text PDFExpanding the Diversity of : A Novel Genus from .
Viruses
January 2025
Biological Sciences Department, University of Pittsburgh, Pittsburgh, PA 15260, USA.
Six novel phages belonging to the family were isolated using as a host. Phages MuffinTheCat, Badulia, DesireeRose, Bee17, SCoupsA, and LuzDeMundo were purified from environmental samples by students participating in the Science Education Alliance Phage Hunters Advancing Genomics and Evolutionary Science (SEA-PHAGES) program at Alliance University, New York. The phages have linear dsDNA genomes 15,438-15,636 bp with 112-120 bp inverted terminal repeats.
View Article and Find Full Text PDFSynthesis of Headful Packaging Phages Through Yeast Transformation-Associated Recombination.
Viruses
December 2024
School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China.
De novo synthesis of phage genomes enables flexible genome modification and simplification. This study explores the synthetic genome assembly of phage vB_PaeS_SCUT-S4 (S4), a 42,932 bp headful packaging phage, which encapsidates a terminally redundant, double-stranded DNA genome exceeding unit length. We demonstrate that using the yeast TAR approach, the S4 genome can be assembled and rebooted from a unit-length genome plus a minimal 60 bp terminal redundant sequence.
View Article and Find Full Text PDFNicking Activity of M13 Bacteriophage Protein 2.
Int J Mol Sci
January 2025
Department of Biomedical Engineering, Faculty of Engineering and Natural Sciences, Acibadem Mehmet Ali Aydinlar University, 34752 Istanbul, Türkiye.
Gene II Protein (Gp2/P2) is a nicking enzyme of the M13 bacteriophage that plays a role in the DNA replication of the viral genome. P2 recognizes a specific sequence at the f1 replication origin and nicks one of the strands and starts replication. This study was conducted to address the limitations of previous experiments, improve methodologies, and precisely determine the biochemical activity conditions of the P2 enzyme in vitro.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!