Formalin-fixed and paraplast-embedded tissue specimens of human pituitary, thyroid, and pancreas were investigated using fluorescamine fluorescence and immunohistochemical methods. Growth hormone-producing cells present in normal and neoplastic pituitary tissue exhibited fluorescamine fluorescence. The other tissues examined showed no fluorescamine binding.

Download full-text PDF

Source
http://dx.doi.org/10.1007/BF00433066DOI Listing

Publication Analysis

Top Keywords

fluorescamine fluorescence
12
growth hormone-producing
8
hormone-producing cells
8
human pituitary
8
fluorescamine
4
fluorescence detection
4
detection growth
4
cells human
4
pituitary adenomas
4
adenomas formalin-fixed
4

Similar Publications

An ecofriendly, effective, and selective spectrofluorimetric approach for natamycin analysis was developed using fluorescamine as a fluorogenic probe. Natamycin is the only topical ocular antifungal medication that is presently on the market for treating keratitis, conjunctivitis, and blepharitis caused by yeast and other fungi. Owing to its primary aliphatic amino group, natamycin can easily interact with fluorescamine resulting in the formation of the highly fluorescent diaryl pyrrolone derivative.

View Article and Find Full Text PDF

Construction of a New Probe Based on Copper Chaperone Protein for Detecting Cu in Cells.

Molecules

February 2024

Laboratory of Protein Based Functional Materials of Shanxi Province, Taiyuan Normal University, Jinzhong 030619, China.

Biomacromolecular probes have been extensively employed in the detection of metal ions for their prominent biocompatibility, water solubility, high selectivity, and easy modification of fluorescent groups. In this study, a fluorescent probe FP was constructed. The probe FP exhibited high specificity recognition for Cu.

View Article and Find Full Text PDF
Article Synopsis
  • Therapeutic drug monitoring (TDM) is essential for ensuring drug safety and effectiveness by measuring drug concentrations in the blood.
  • A novel microfluidic device was developed to purify blood samples, enabling the detection of tamsulosin, a medication for benign prostatic hyperplasia, after converting it into a detectable fluorescent form.
  • The device demonstrated high precision and reliability with a low cost, making it a practical point-of-care solution that could improve patient care and broaden access to therapeutic monitoring.
View Article and Find Full Text PDF

One-pot derivatization/magnetic solid-phase extraction coupled with liquid chromatography-fluorescence detection for the rapid determination of sulfonamide residues in honey.

Food Chem X

March 2024

Key Laboratory of Targeting Therapy and Diagnosis for Critical Diseases of Henan Province, and School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, China.

Consuming foods with excess sulfonamide residues threatens human health, underscoring the importance of their detection in food. This study presents an innovative one-pot derivatization/magnetic solid-phase extraction (OPD/MSPE) method for sulfonamides analysis. This approach integrates the derivatization and extraction steps into a single process.

View Article and Find Full Text PDF

Investigating fluorogenic labelling of amine coupled with first-order derivative spectrofluorimetry as a versatile analytical methodology: Application to estimation of benoxinate in its eye drops.

Spectrochim Acta A Mol Biomol Spectrosc

January 2024

Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt; Pharmaceutical Chemistry Department, Faculty of Pharmacy, Delta University for Science and Technology, Gamasa 35712, Egypt.

In this research, fluorogenic labelling followed by applying first-order derivative spectrofluorimetry for the developed fluorophore is discussed as an alternative, sensitive and selective analytical approach. Benoxinate, containing a primary amine and fluorescamine reagent were selected for the study. Then the proposed methodology relies on the reaction between the primary amine in benoxinate with fluorescamine that selectively reacts with primary amines to develop highly fluorescent products.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!