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In Silico Exploration of Staphylococcal Cassette Chromosome (SCC) Evolution Based on Phylogenetic Relationship of .
Microorganisms
January 2025
College of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China.
As the mobile cassette carrier of the methicillin resistance gene that is transported across staphylococci species, the evolution and origin of Staphylococcal Cassette Chromosome (SCC)-and in particular, the composition of and SCC-have been extensively discussed in the scientific literature; however, information regarding its dissemination across geographical limits and evolution over decades remains limited. In addition, whole-genome sequencing-based macro-analysis was unable to provide sufficiently detailed evolutionary information on SCC. Herein, the cassette chromosome recombinase genes , as essential components of SCC, were employed to explore the evolution of SCC.
View Article and Find Full Text PDFQualitative evaluation of the implementation and national roll-out of the NHS App in England.
BMC Med
January 2025
Nuffield Department of Primary Care Health Sciences, University of Oxford, Radcliffe Observatory Quarter, Woodstock Road, Oxford, OX2 6GG, UK.
Background: The NHS App launched in 2019 as the 'digital front door' to the National Health Service in England with core features including General Practitioner (GP) appointment booking, repeat prescriptions, patient access to records and, later on, COVID-19 vaccination certification. Similar patient portals have been adopted in different formats and with variable levels of success. In this longitudinal study (2021-2023) we examined how the NHS App became implemented in the pandemic context and beyond.
View Article and Find Full Text PDFSUMMER: an integrated nanopore sequencing pipeline for variants detection and clinical annotation on the human genome.
Funct Integr Genomics
January 2025
Children's Medical Center, Peking University First Hospital, No.5 Le Yuan Road, Daxing District, 100034, Beijing, China.
Long-read sequencing has emerged as a transformative technology in recent years, offering significant potential for the molecular diagnosis of unresolved genetic disorders. Despite its promise, the comprehensive detection and clinical annotation of genomic variants remain intricate and technically demanding. We present SUMMER, an integrated and structured workflow specifically designed to process raw Nanopore sequencing reads.
View Article and Find Full Text PDFAssociation of Mobile Stroke Unit Care and Spending, Utilization, and Death in New York City.
J Am Heart Assoc
January 2025
Department of Population Health Sciences Weill Cornell Medicine New York NY.
Background: Transport by mobile stroke units (MSUs), which provide access to computed tomography scanning and intravenous blood pressure medications and thrombolytics, reduces time to treatment and may improve short-term functional outcomes for patients with acute stroke. The longer-term clinical and financial impacts remain incompletely understood. The aim of the study was to determine whether MSU care is associated with better health, utilization, and spending outcomes for patients with suspected acute stroke.
View Article and Find Full Text PDFValidity Analysis of Monocular Human Pose Estimation Models Interfaced with a Mobile Application for Assessing Upper Limb Range of Motion.
Sensors (Basel)
December 2024
Postgraduate Program in Biotechnology, Parnaíba Delta Federal University, Parnaíba 64202-020, Brazil.
Human Pose Estimation (HPE) is a computer vision application that utilizes deep learning techniques to precisely locate Key Joint Points (KJPs), enabling the accurate description of a person's pose. HPE models can be extended to facilitate Range of Motion (ROM) assessment by leveraging patient photographs. This study aims to evaluate and compare the performance of HPE models for assessing upper limbs ROM.
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