The present state of studies on prediction of the chromatographic and electrophoretic behaviour of oligonucleotides is reviewed; particular emphasis is given to high-performance liquid chromatographic and capillary electrophoretic separations. Attention is paid to fundamental theory for the prediction of retention and migration times, and bandwidths. The article also deals with the applicability of the theory to the computer-assisted prediction and the computer simulation for these two types of separation of oligonucleotides. Optimization of separation conditions using the computer simulation system is briefly described.
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