Based on the notion that regions of structural genes which encode critical domains of the corresponding proteins are highly conserved among closely related species, oligonucleotide primers were designed and used to amplify the alpha-globin sequence(s) of the Sprague-Dawley (SD) rat. Data of these amplified sequence constructs showed that two new rat alpha-globin specific sequences have been identified. Southern hybridization confirmed the presence of these sequences in the rat genome.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.3109/03630269308997489 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Relaxin suppresses atrial fibrillation, reverses fibrosis and reduces inflammation in aged hearts.
Biochem Pharmacol
September 2024
Departments of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Healthy aging results in cardiac structural and electrical remodeling that increase susceptibility to cardiovascular diseases. Relaxin has shown broad cardioprotective effects including anti-fibrotic, anti-arrhythmic and anti-inflammatory outcomes in multiple models. This paper focuses on the cardioprotective effects of Relaxin in a rat model of aging.
View Article and Find Full Text PDFIn-vitro study of the radioprotective effect of palladium α-lipoic acid nano-complex on hemoglobin molecule.
Spectrochim Acta A Mol Biomol Spectrosc
September 2021
Molecular Biophysics Lab, Radiation Physics Depart, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority, POB 29 Madinat Nasr, Cairo, Egypt. Electronic address:
This work is intended to study the radioprotective effect of palladium α-lipoic acid nano-complex (PLAC) on hemoglobin molecule in vitro. Blood samples were obtained from adult male rats weighing 120-150 g after dissection, using heparinized needles. Each blood sample was divided into four groups; the first group was kept untreated as control, palladium α-lipoic acid (PLAC) was added to the second group at concentration 2% v/v, the third group was exposed to 100 Gy gamma radiation and the forth group was irradiated with the addition of PLAC.
View Article and Find Full Text PDFUnannotated single nucleotide polymorphisms in the TATA box of erythropoiesis genes show in vitro positive involvements in cognitive and mental disorders.
BMC Med Genet
October 2020
Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10 Lavrentyev Ave, Novosibirsk, 630090, Russia.
Background: Hemoglobin is a tetramer consisting of two α-chains and two β-chains of globin. Hereditary aberrations in the synthesis of one of the globin chains are at the root of thalassemia, one of the most prevalent monogenic diseases worldwide. In humans, in addition to α- and β-globins, embryonic zeta-globin and fetal γ-globin are expressed.
View Article and Find Full Text PDFOrigin and Evolution of the Gene Family of Proteinaceous Pheromones, the Exocrine Gland-Secreting Peptides, in Rodents.
Mol Biol Evol
January 2021
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
The exocrine-gland secreting peptide (ESP)gene family encodes proteinaceous pheromones that are recognized by the vomeronasal organ in mice. For example, ESP1 is a male pheromone secreted in tear fluid that regulates socio-sexual behavior, and ESP22 is a juvenile pheromone that suppresses adult sexual behavior. The family consists of multiple genes and has been identified only in mouse and rat genomes.
View Article and Find Full Text PDFSystemic and microvascular comparison of Lactated Ringer's solution, VIR-HBOC, and alpha-alpha crosslinked haemoglobin-based oxygen carrier in a rat 10% topload model.
Artif Cells Nanomed Biotechnol
December 2020
Song Biotechnologies LLC, Baltimore, MD, USA.
Medical support for traumatic haemorrhage is lacking for far-forward combat units. VIR-HBOC (haemoglobin-based oxygen carrier) is a novel biological therapeutic under development as a field-stable resuscitation fluid. HBOCs have a long history of complications, chief among them is vasoconstrictive hypertension, which must be resolved before efficacy testing.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!