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A novel strategy to improve the thermostability of Penicillium camembertii mono- and di-acylglycerol lipase.
Biochem Biophys Res Commun
June 2018
School of Food Science and Engineering, Guangdong Research Center of Lipid Science and Applied Engineering Technology, South China University of Technology, Guangzhou, 510641, PR China. Electronic address:
Penicillium camembertii (PCL), a mono- and di-acylglycerol lipase (DGL), has the vital potential in the oil chemistry for food industry. However, known DGLs are mesophilic enzymes which restricts its application in the industry. To improve thermostability of PCL, we used amino acid substitution by comparison of amino acids compositions of PCL and protein sequences from typical thermophilic bacteria.
View Article and Find Full Text PDFMalassezia globosa MgMDL2 lipase: Crystal structure and rational modification of substrate specificity.
Biochem Biophys Res Commun
June 2017
School of Food Science and Engineering, South China University of Technology, Guangzhou, PR China. Electronic address:
Lipases play an important role in physiological metabolism and diseases, and also have multiple industrial applications. Rational modification of lipase specificity may increase the commercial utility of this group of enzymes, but is hindered by insufficient mechanistic understanding. Here, we report the 2.
View Article and Find Full Text PDFA mechanistic study into the epoxidation of carboxylic acid and alkene in a mono, di-acylglycerol lipase.
Biochem Biophys Res Commun
May 2015
College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, PR China. Electronic address:
More and more industrial chemistry reactions rely on green technologies. Enzymes are finding increasing use in diverse chemical processes. Epoxidized vegetable oils have recently found applications as plasticizers and additives for PVC production.
View Article and Find Full Text PDFBiochemical properties of a new cold-active mono- and diacylglycerol lipase from marine member Janibacter sp. strain HTCC2649.
Int J Mol Sci
June 2014
College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
Mono- and di-acylglycerol lipase has been applied to industrial usage in oil modification for its special substrate selectivity. Until now, the reported mono- and di-acylglycerol lipases from microorganism are limited, and there is no report on the mono- and di-acylglycerol lipase from bacteria. A predicted lipase (named MAJ1) from marine Janibacter sp.
View Article and Find Full Text PDFEnzymatic synthesis of extremely pure triacylglycerols enriched in conjugated linoleic acids.
Molecules
August 2013
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China.
This work was objectively targeted to synthesize extremely pure triacylglycerols (TAG) enriched in conjugated linoleic acids (CLAs) for medical and dietetic purposes. Extremely pure CLA-enriched TAG was successfully synthesized by using the multi-step process: TAG was primarily synthesized by lipase-catalyzed esterification of CLA and glycerol and then the lower glycerides [monoacylglycerol (MAG) and diacylglycerol (DAG)] in the esterification mixtures was hydrolyzed to free fatty acids (FFAs) by a mono- and di-acylglycerol lipase (lipase SMG1), finally, the FFAs were further separated from TAG by low temperature (150 °C) molecular distillation. The operation parameters for the lipase SMG1-catalyzed hydrolysis were optimized using response surface methodology based on the central composite rotatable design (CCRD).
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