Use of nonradioactive DNA probes to identify a Campylobacter jejuni strain causing abortion.

Eur J Clin Microbiol Infect Dis

Département de Microbiologie Médicale et Moleculaire, Centre Hospitalier Universitaire Bretonneau, Tours, France.

Published: August 1993

A case of human abortion due to a Campylobacter infection is reported. Cultures revealed two morphologically different isolates with large and small colonies respectively. Using conventional methods of identification, the large colonies were identified as Campylobacter jejuni and the small colonies as Campylobacter coli. Dot blot hybridization and determination of rDNA restriction fragment patterns revealed that both colony types were the same strain of Campylobacter jejuni. This observation illustrates the need to use methods other than phenotypic methods when identifying strains of Campylobacter.

Download full-text PDF

Source
http://dx.doi.org/10.1007/BF01973644DOI Listing

Publication Analysis

Top Keywords

campylobacter jejuni
12
small colonies
8
campylobacter
6
nonradioactive dna
4
dna probes
4
probes identify
4
identify campylobacter
4
jejuni strain
4
strain causing
4
causing abortion
4

Similar Publications

The Campylobacter prevalence in free-ranging broiler flocks is usually higher than in conventional flocks, and effective interventions for this production type are needed. This study aimed to investigate the on-farm Campylobacter-reducing effect of feeding three feed additives or a water additive to broilers from hatching to slaughter. Newly hatched Ranger Gold broilers (n = 140) were randomly placed into five cages (n = 28/cage) within a flock of 6,000 broilers.

View Article and Find Full Text PDF

Holistic monitoring of Campylobacter jejuni biofilms with NanoLuc bioluminescence.

Appl Microbiol Biotechnol

December 2024

Biotechnical Faculty, Department of Food Science and Technology, University of Ljubljana, Ljubljana, Slovenia.

Campylobacter jejuni, a major cause of foodborne zoonotic infections worldwide, shows a paradoxical ability to survive despite its susceptibility to environmental and food-processing stressors. This resilience is likely due to the bacterium entering a viable but non-culturable state, often within biofilms, or even initiating biofilm formation as a survival strategy. This study presents an innovative application of NanoLuc bioluminescence to accurately monitor the development of C.

View Article and Find Full Text PDF

is a serious health threat because of the rapid progressive evolution of antimicrobial resistance and efficient transmission from zoonotic as well as human sources. Resistance to fluoroquinolones and macrolides is particularly concerning as this compromises the two most effective oral antibiotic agents currently available for human campylobacteriosis. Here, we report on the prevalence and worldwide distribution of the operon , which encodes an efflux pump conferring high levels of combined resistance to fluoroquinolones and macrolides in strains isolated from poultry ( = 75) and children ( = 177).

View Article and Find Full Text PDF

Due to the inaccessibility of β1-4-N-acetylgalactosaminyltransferase for the direct glycan chain elongation, the enzymatic synthesis of 0-series ganglioside with extended backbone has not been explored. In this the sialic acid was enzymatically introduced as an auxiliary group to overcome the limitation of substrate specificity of Campylobacter jejuni β1-4-N-acetylgalactosaminyltransferase (CjCgtA) to achieve the synthesis of desired extended 0-series ganglioside core structures. A bacterial α2-6-sialyltransferase from Photobacterium damselae (Pd2,6ST) exhibits unexpected acceptor substrate specificity for 0-series ganglioside core structures, providing an easy access for the synthesis of complex gangliosides bearing the sialyl N-acetylgalactosamine unit.

View Article and Find Full Text PDF

An Atypical Case of Miller Fisher Syndrome.

Cureus

November 2024

Internal Medicine, Summa Health, Akron, USA.

Miller Fisher syndrome (MFS) is a rare variant of Guillain-Barré syndrome (GBS) characterized by a classic triad of external ophthalmoplegia, ataxia, and areflexia, often following a recent infection.  Understanding atypical presentations of MFS is crucial for timely diagnosis and management, as the syndrome may be mistaken for other neurological disorders. This report aims to highlight the clinical journey of the patient, including symptom onset, diagnostic challenges, and therapeutic interventions, with a discussion of the broader implications of such atypical cases in the context of MFS.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!