AI Article Synopsis
- Experiments were conducted on anesthetized rabbits to study the localization of horseradish peroxidase (HRP)-labelled neurons in the motor nucleus of the trigeminal nerve, showing significant clustering in the caudal region.
- These HRP-labelled neurons potentially control muscles in the upper respiratory airways while also connecting to central respiratory neurons, suggesting a dual role in regulating breathing.
- The findings suggest that these neurons may help synchronize the upper and lower respiratory systems, contributing to overall respiratory rhythm regulation.
Article Abstract
Experiments were performed with anaesthetized (urethan and chloralose) spontaneously breathing rabbits. Horseradish peroxidase (HRP) was injected (1 mg) with a Hamilton syringe into the end of the sectioned mylohyoid nerve. The HRP-labelled neurones could be localized in the motor nucleus of the trigeminal nerve (N.V.mt.). It was found that the labelled neurones are present in the caudal and intermediate part of the nucleus. However, their largest agglomerations were noted in the caudal part, where they occupied the entire frontal section of the nucleus. It is assumed that the N.V.mt. directing their axons to the muscles of the upper respiratory airways, simultaneously send collaterals to the central respiratory neurones participating in the regulation of the respiratory rhythm. According to this hypothesis the neurones of the N.V.mt. may ensure synchronisation between the upper and lower respiratory system.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Tyramine-enzyme conjugate repeats for an interdigitated capacitance immunosensing array in the detection of neuroblastoma biomarker neuron-specific enolase.
Analyst
January 2025
Department of Pediatric Surgery, The First Affiliated Hospital of Fujian Medical University, 20 Cha-Zhong Road, Taijiang District, Fuzhou 350005, China.
Methods based on enzyme labelling strategies have been widely developed for capacitance immunoassays, but most suffer from low sensitivity and are unfavorable for routine use in the early stages of diagnostics. Herein, we designed a highly efficient capacitance immunosensing method for the low-abundance neuroblastoma biomarker neuron-specific enolase (NSE) using an interdigitated micro-comb electrode. Initially, monoclonal mouse anti-human NSE capture antibodies were immobilized on the interdigitated gold electrodes using bovine serum albumin.
View Article and Find Full Text PDFCofactor-directed co-immobilization of dual-enzyme on functionalized montmorillonite with enhanced catalytic performance.
Int J Biol Macromol
January 2025
School of Environment and Resource, Key Laboratory of Solid Waste Treatment and Resource Recycle of Ministry of Education, Southwest University of Science and Technology, Mianyang, Sichuan 621010, China.
Recently, multi-enzyme cascade catalysis has attracted increasing attention due to the advantages of integrating multiple enzymes, few side reactions and high catalytic efficiency. Herein, a novel dual-enzyme cascade system (GOx-FMt-HRP) was developed through cofactor-directed orientational co-immobilization of glucose oxidase (GOx) and horseradish peroxidase (HRP) onto functional montmorillonite (FMt). The presented method realizes the reconstitution of cofactors and apo-enzymes (enzymes without cofactors), which enables enzymes to be immobilized in specific orientations on the support, thereby effectively reducing changes in their conformation.
View Article and Find Full Text PDFGlucose-triggered NIR-responsive photothermal antibacterial gelatin/dextran hydrogel simultaneously targeting the high glucose and infection microenvironment in diabetic wound.
Int J Biol Macromol
January 2025
School of Life Science, Zhengzhou University, 100 Science Road, Zhengzhou 450001, China. Electronic address:
The treatment of diabetic wounds with bacterial infection is a major challenge in the medical field. Microenvironment-responsive hydrogel dressings have shown great advantages, and photothermal antibacterial therapy is a potential antimicrobial strategy to avoid the generation of resistant bacteria. In this work, a glucose-triggered near-infrared (NIR)-responsive photothermal antibacterial hydrogel was designed and named GOGD based on a cascade reaction of glucose oxidation and polyphenol polymerization.
View Article and Find Full Text PDFCoproantigen detection and molecular identification of Cryptosporidium species among newborn and adult farm animals.
AMB Express
January 2025
Parasitology and Animal Diseases Department, Veterinary Research Institute, National Research Centre, El Buhouth St., Dokki, Giza, Egypt.
Cryptosporidium sp. is an obligatory intracellular apicomplexan protozoan parasite that causes a disease called cryptosporidiosis with substantial veterinary and medical importance. Therefore, this study aimed to evaluate an early diagnosis of cryptosporidiosis using the anti-Cryptosporidium parvum oocyst immunoglobulin IgG polyclonal antibodies (anti-C.
View Article and Find Full Text PDFCombining Hard Shell with Soft Core to Enhance Enzyme Activity and Resist External Disturbances.
Adv Sci (Weinh)
January 2025
Department of Cardiology, The First People's Hospital of Wenling, Wenling Hospital of Wenzhou Medical University, Wenling, Zhejiang, 317500, China.
Immobilizing enzymes onto solid supports having enhanced catalytic activity and resistance to harsh external conditions is considered as a promising and critical method of broadening enzymatic applications in biosensing, biocatalysis, and biomedical devices; however, it is considerably hampered by limited strategies. Here, a core-shell strategy involving a soft-core hexahistidine metal assembly (HmA) is innovatively developed and characterized with encapsulated enzymes (catalase (CAT), horseradish peroxidase, glucose oxidase (GOx), and cascade enzymes (CAT+GOx)) and hard porous shells (zeolitic imidazolate framework (ZIF), ZIF-8, ZIF-67, ZIF-90, calcium carbonate, and hydroxyapatite). The enzyme-friendly environment provided by the embedded HmA proves beneficial for enhanced catalytic activity, which is particularly effective in preserving fragile enzymes that will have been deactivated without the HmA core during the mineralization of porous shells.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!