AI Article Synopsis
Article Abstract
Several peptidic and non-peptidic factors can modulate Na,K-ATPase activity, among them mainly inhibitors of this enzyme, ouabain being the most effective. In a very few cases only, activation of Na,K-ATPase by endogenous factors has been recorded. We have investigated the effect on Na,K-ATPase of a novel regulatory peptide, PEC-60, recently isolated from porcine intestine. Various biological effects have been described for PEC-60 in different tissues, including brain. We have found that PEC-60 caused a dose-dependent activation of Na,K-ATPase from rat brain frontal cortex, whereas the carboxymethylated form of PEC-60 or other hormonal peptides had no effect. The maximal value of activity reaches up to 125% at close to micromolar concentrations of PEC-60 and the dependence can be described with a bell-shaped curve, indicating a complex mechanism for the interaction. The activation of the enzyme by PEC-60 is apparently related to Na(+)-dependent steps of the Na,K-ATPase system. The kinetic parameters for K(+)-phosphatase were unaffected. Moreover, the activating effect was enhanced by preincubation at low concentrations of ATP that transform the enzyme into the Na(+)-form. Due to the crucial physiological role of Na,K-ATPase, its activity has to be finely controlled and thus PEC-60 may be one of the endogenous factors that regulate this enzyme.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0014-5793(94)00407-2 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Disrupting Na ion homeostasis and Na/K ATPase activity in breast cancer cells directly modulates glycolysis in vitro and in vivo.
Cancer Metab
May 2024
School of Biomedical Engineering and Imaging Sciences, King's College London, St Thomas' Hospital, London, SE1 7EH, UK.
Article Synopsis
- Cancer cells need more energy (ATP) to grow and survive, which can change how they take in and balance sodium ions in their bodies.
- The researchers used special methods to measure sodium levels and how fast cancer cells convert sugar into energy, comparing cancer cells to normal cells to see the differences.
- They found that when they blocked a specific pump that controls sodium balance (Na/K-ATPase), the cancer cells had more sodium and produced less energy, showing that sodium levels affect their energy production.
High dietary lipid level promotes low salinity adaptation in the marine euryhaline crab ().
Anim Nutr
March 2023
Laboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China.
The physiological processes involved in adaptation to osmotic pressure in euryhaline crustaceans are highly energy demanding, but the effects of dietary lipids (fat) on low salinity adaptations have not been well evaluated. In the present study, a total of 120 mud crabs (, BW = 17.87 ± 1.
View Article and Find Full Text PDFR125H, W240S, C386R, and V507I SLC4A11 mutations associated with corneal endothelial dystrophy affect the transporter function but not trafficking in PS120 cells.
Exp Eye Res
March 2019
School of Optometry, Indiana University Bloomington, Bloomington, IN, USA. Electronic address:
Article Synopsis
- SLC4A11 mutations are linked to various corneal dystrophies and associated conditions like Harboyan syndrome.
- Research on mice missing the Slc4a11 gene shows they develop issues similar to congenital hereditary endothelial dystrophy, emphasizing the protein's role in corneal health.
- Five specific SLC4A11 mutations were analyzed in a cell line, revealing that, while there were no significant differences in protein levels at the cell surface, all mutations led to reduced acidification and H ion currents compared to the wild type, indicating altered transport properties rather than protein trafficking issues.
Alzheimer's Toxic Amyloid Beta Oligomers: Unwelcome Visitors to the Na/K ATPase alpha3 Docking Station.
Yale J Biol Med
March 2017
Department of Neurobiology, Weinberg College of Arts & Sciences, Northwestern University; Department of Neurology, Feinberg School of Medicine, Northwestern University.
Toxic amyloid beta oligomers (AβOs) are known to accumulate in Alzheimer's disease (AD) and in animal models of AD. Their structure is heterogeneous, and they are found in both intracellular and extracellular milieu. When given to CNS cultures or injected ICV into non-human primates and other non-transgenic animals, AβOs have been found to cause impaired synaptic plasticity, loss of memory function, tau hyperphosphorylation and tangle formation, synapse elimination, oxidative and ER stress, inflammatory microglial activation, and selective nerve cell death.
View Article and Find Full Text PDFLow dose ouabain stimulates NaK ATPase α1 subunit association with angiotensin II type 1 receptor in renal proximal tubule cells.
Biochim Biophys Acta
November 2016
Department of Physiology and Biophysics, Howard University College of Medicine, Washington, DC, USA. Electronic address:
Our laboratory has recently demonstrated that low concentrations of ouabain increase blood pressure in rats associated with stimulation of NaK ATPase activity and activation of the Src signaling cascade in NHE1-dependent manner. Proteomic analysis of human kidney proximal tubule cells (HKC11) suggested that the Angiotensin II type 1 receptor (AT1R) as an ouabain-associating protein. We hypothesize that ouabain-induced stimulation of NaK ATPase activity is mediated through AT1R.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!