AI Article Synopsis
- The study investigated the cytotoxic effects of halothane on isolated rat liver cells (hepatocytes) using electron microscopy and cell viability assessments.
- Soon after exposure to lower concentrations (0.5% and 0.75%), significant changes in the plasma membrane were observed, which reduced cell viability to 70%.
- At higher concentrations (1% and 3%), mitochondrial degeneration occurred, further decreasing cell viability to 40%, indicating that halothane impacts cell structure and function in a dose-dependent manner.
Article Abstract
To determine the direct cytotoxicity of halothane for liver cells, the effects of this agent on isolated rat hepatocytes were investigated by electron microscopy and by examining cell viability. Soon after treatment with lower concentrations of halothane (0.5% and 0.75%), there were marked changes in the plasma membrane. Cell viability was reduced to 70% of the control. At higher concentrations of halothane (1% and 3%), degeneration of mitochondria was noted. Cell viability was reduced to 40% of the control. Halothane, at lower concentrations, appeared to induce early morphological alterations of the plasma membrane, while higher concentrations damaged the organella.
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