A crude lysozyme preparation was recovered in waste from the scallop processing industry. Lysozyme was then purified 229-fold in preparative scale by chromatography on S Sepharose and Blue Sepharose. Further purification on Sephacryl S-200 resulted in a lysozyme preparation with a specific activity of 64,000 units/mg protein. The apparent molecular mass of the partially purified lysozyme was 10 kDa as judged by gel filtration. Optimum pH for lysis of Micrococus luteus under the present conditions was 5.2. The enzyme was very active at low temperatures. At 4 degrees C the scallop viscera lysozyme exhibits about 55% of the activity measured at 37 degrees C.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/10826069408010083 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Preparation of halloysite nanotube-based monolithic column for small molecules and protein analysis.
J Chromatogr B Analyt Technol Biomed Life Sci
January 2025
College of Life Science, Hebei Agricultural University, Baoding, Hebei 071001, China; Hebei Forage Microbial Technology Innovation Center, Baoding, Hebei 071001, China; Hebei Agriculture Waste Resource Utilization Engineering Research Center, Baoding, Hebei 071001, China. Electronic address:
s: This study aimed to prepare a new separation medium, silane coupling agent KH570- modified halloysite nanotube (MPS-HNT) monolithic column, with excellent separation performance for small molecular compounds and macromolecular proteins. This was prepared using the principle of redox polymerization with modified HNTs as monomers. The optimal monomer proportion was obtained by optimizing the ratio of monomer, cross-linker, and pore-forming agent, which was evaluated using scanning electron microscopy, nitrogen adsorption, and mercury intrusion.
View Article and Find Full Text PDFInjectable in situ forming hydrogel based on carboxymethyl chitosan for sustained release of hyaluronic acid: A viscosupplement for biomedical applications.
Carbohydr Polym
March 2025
Orthopaedic clinic, Mehrad hospital, Tehran, Iran.
The reduction in hyaluronic acid concentration and viscosity in the synovial fluid of patients struggling with osteoarthritis increases the abrasion of articular cartilage. The aim of this study was to design a semi-IPN hydrogel based on genipin-crosslinked carboxymethyl chitosan (CMCh) and glycerol to achieve long-term release of hyaluronic acid. The results showed that hydrogel comprising CMCh (3 % wt.
View Article and Find Full Text PDFA phase-transited lysozyme coating doped with strontium on titanium surface for bone repairing via enhanced osteogenesis and immunomodulatory.
Front Cell Dev Biol
January 2025
Hunan Cancer Hospital, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, China.
Introduction: Titanium is currently recognized as an excellent orthopedic implant material, but it often leads to poor osseointegration of the implant, and is prone to aseptic loosening leading to implant failure. Therefore, biofunctionalization of titanium surfaces is needed to enhance their osseointegration and immunomodulation properties to reduce the risk of implant loosening. We concluded that the utilization of PTL-Sr is a direct and effective method for the fabrication of multifunctional implants.
View Article and Find Full Text PDFLayer-by-layer coated probiotics with tannic acid-Ca and casein phosphopeptide complexes for caries prevention and enamel remineralization.
iScience
January 2025
Department of Orthodontics, Tianjin Medical University School and Hospital of Stomatology & Tianjin Key Laboratory of Oral Soft and Hard Tissues Restoration and Regeneration, No.12 Qixiangtai Road, Heping District, Tianjin 300070, P.R. China.
Dental caries is a common disease resulting from tooth demineralization caused by bacterial plaque. Probiotics have shown great potential against caries by regulating the balance of oral flora. However, obstacles such as poor colonization and lysozyme sensitivity in oral cavity hinder their further application.
View Article and Find Full Text PDFConstruction of Pickering Double Emulsions Based on Xanthan Gum/Lysozyme Nanoparticles: Structure, Stability, and Co-Encapsulation of Epigallocatechin Gallate and β-Carotene.
Foods
January 2025
College of Life Science, Xinyang Normal University, Xinyang 464000, China.
The low stability of water-in-oil-in-water (W/O/W) double emulsions greatly limits their applications. Therefore, in this study, W/O/W Pickering double emulsions (PDEs) were prepared by a two-step emulsification method using polyglycerol polyricinoleate (PGPR) and xanthan gum/lysozyme nanoparticles (XG/Ly NPs) as lipophilic and hydrophilic emulsifiers, respectively. The regulation mechanism of the performance of PDEs by XG/Ly NPs was investigated, and the ability of the system to co-encapsulate epigallocatechin gallate (EGCG) and β-carotene was evaluated.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!