Proteolytic activity of 238 strains of Candida was investigated by the Staib method in Rózga modification, permitting for quantitative evaluation of the result (agar medium with albumin). Analyzing size of proteolysis zones--obtained for strains isolated from selected ontocenoses of the same women--a linear correlation coefficient was used; for confirmation of identity of tested strains a conformity coefficient was used. Isolated strains were identified as C. albicans, C. guilliermondii, C. krusei, C. pseudotropicalis, C. tropicalis and other species (singular). It was found that the curve of distribution of proteolysis zone sizes of all strains is normal and that statistically significant correlation between size of proteolysis zone of strains isolated from vagina and oral cavity of the same women does exist.
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Ecotoxicol Environ Saf
January 2025
Key laboratory of Birth Defects and Related Disease of Women and Children (Sichuan University), Ministry of Education, Chengdu, Sichuan, China; SCU-CUHK Joint Laboratory for Reproductive Medicine, Zebrafish Research Platform, West China Second University Hospital, Children's Medicine Key Laboratory of Sichuan Province, Sichuan University/Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu 610000, PR China. Electronic address:
Noise pollution has become a significant concern for human health, yet its effects on early embryonic development remain underexplored. Specifically, data on the impact of sine wave noise on newly fertilized embryos is limited. This study aimed to address this gap by using zebrafish embryos at the 1-cell stage as a model to assess the toxicity of sine waves, following OECD Test No.
View Article and Find Full Text PDFJ Control Release
January 2025
Department of Pharmacy, University of Copenhagen, Universitetsparken 2, Copenhagen 2100, Denmark; Bioneer A/S, Kogle Allé 2, Hørsholm 2970, Denmark. Electronic address:
The ability to predict the absorption of exenatide (Ex), a GLP-1 analogue, after oral dosing to rats in self-nanoemulsifying drug delivery systems (SNEDDS), using in vitro methods, was assessed. Ex was complexed with soybean phosphatidylcholine (SPC) prior to loading into SNEDDS. A design of experiments (DoE) approach was employed to develop SNEDDS incorporating medium-chain triglycerides (MCT), medium-chain mono- and diglycerides (MGDG), Kolliphor® RH40, and monoacyl phosphatidylcholine.
View Article and Find Full Text PDFbioRxiv
January 2025
Ben-May Institute for Cancer Research, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.
Insulin degrading enzyme (IDE) is a dimeric 110 kDa M16A zinc metalloprotease that degrades amyloidogenic peptides diverse in shape and sequence, including insulin, amylin, and amyloid-β, to prevent toxic amyloid fibril formation. IDE has a hollow catalytic chamber formed by four homologous subdomains organized into two ~55 kDa N- and C- domains (IDE-N and IDE-C, respectively), in which peptides bind, unfold, and are repositioned for proteolysis. IDE is known to transition between a closed state, poised for catalysis, and an open state, able to release cleavage products and bind new substrate.
View Article and Find Full Text PDFCancers (Basel)
December 2024
Department of Clinical and Biological Sciences, University of Torino, 10043 Turin, Italy.
Background And Aims: Cancer cachexia is a complex syndrome affecting most cancer patients and is directly responsible for about 20% of cancer-related deaths. Previous studies showed muscle proteolysis hyper-activation and mitophagy induction in tumor-bearing animals. While basal mitophagy is required for maintaining muscle mass and quality, excessive mitophagy promotes uncontrolled protein degradation, muscle loss and impaired function.
View Article and Find Full Text PDFCell Biol Toxicol
January 2025
Department of General Surgery, Shengjing Hospital of China Medical University, 36 Sanhao Street, 110004, Shenyang, China.
Background: RING finger protein 112 (RNF112) exerts a key role in human tumors. However, its biological function in colorectal cancer (CRC) has not been discussed. We aimed to explore the function and molecular mechanism of RNF112 in CRC.
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