A single tube reverse transcription-polymerase chain reaction (RT-PCR) method for detection of African horse sickness virus (AHSV) in splenic tissues from infected horses is described. Double stranded RNA was extracted from infected organs of horses and used to produce complementary DNA (cDNA) with the two primers selected for the PCR. The 1179 bp amplified product (the segment 7 which encodes for VP 7), detected by electrophoresis on agarose gel and ethidium bromide staining, was hydrolysed with eight restriction endonucleases for characterization of the AHSV. The sensitivity of this method is discussed. Application of the RT-PCR method should improve detection and shorten the time required to confirm a clinical diagnosis of AHSV infection.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0166-0934(94)90102-3 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Factors influencing equine veterinarians' job satisfaction and retention: A focus group study.
Equine Vet J
January 2025
Richard A. Gillespie College of Veterinary Medicine, Lincoln Memorial University, Harrogate, Tennessee, USA.
Background: There is a shortage of equine veterinarians. Understanding what factors are associated with job satisfaction in equine veterinarians can inform interventions to increase retention in equine medicine.
Objective: To explore the prominent factors causing work dissatisfaction and burnout in equine veterinarians.
A polygeneric immunogen composed of 22 venoms from sub-Saharan African snakes to expand the neutralization scope of the EchiTAb-plus-ICP antivenom.
Toxicon X
December 2024
Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.
Recent research suggests that a polygeneric immunogen made from the venoms of the most medically important viperid and elapid snakes in sub-Saharan Africa could elicit a broader antibody response in horses compared to the current EchiTAb-plus-ICP antivenom, especially against neurotoxic elapid venoms. To test this, 25 horses that have been regularly immunized to produce this antivenom were reimmunized with an immunogen containing 22 venoms from various snake species from the genera , , , and both spitting and non-spitting . The plasma collected from these horses was processed using the caprylic acid method to produce an industrial-scale freeze-dried antivenom.
View Article and Find Full Text PDFFive epidemiological aspects of ASF were evaluated using literature reviews, field studies, questionnaires and mathematical models. First, a literature review and a case-control study in commercial pig farms emphasised the importance of biosecurity and farming practices, including the spread of manure around farms and the use of bedding material as risk factors, while the use of insect nets was a protective factor. Second, although wild boar density is a relevant known factor, the statistical and mechanistic models did not show a clear and consistent effect of wild boar density on ASF epidemiology in the selected scenarios.
View Article and Find Full Text PDFIdentification and Characterization of Linear Epitopes of Monoclonal Antibodies Against African Horse Sickness Virus Serotype 1 VP2 Protein.
Viruses
November 2024
State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150069, China.
African horse sickness (AHS) is an acute, fatal, contagious disease of animals of the family Equidae and is caused by infection with the African horse sickness virus (AHSV). Based on the outer capsid protein VP2, AHSV is classified into nine serotypes (AHSV-1 to -9) with little or no serological cross-reactivity between them. In 2020, AHS outbreaks caused by AHSV-1 were reported in Thailand and Malaysia, marking the first occurrences of AHS in Southeast Asia.
View Article and Find Full Text PDFInsights into the virome of Hyalomma marginatum in the Danube Delta: a major vector of Crimean-Congo hemorrhagic fever virus in Eastern Europe.
Parasit Vectors
November 2024
Pathogen Discovery Laboratory, Institut Pasteur, Université Paris Cité, 75015, Paris, France.
Background: Ticks are significant vectors of pathogens, including viruses, bacteria, and protozoa. With approximately 900 tick species worldwide, many are expanding their geographical range due to changing socioeconomic and climate factors. The Danube Delta, one of Europe's largest wetlands, is an ecosystem that, despite its ecological importance, remains understudied concerning the risk of introducing new tick-borne viruses.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!