Satellite cells were isolated from human quadriceps and masseter muscles and the phenotype of these cells examined in vitro. The expression of the different isoforms of the myosin heavy chains (embryonic, fetal, fast and slow) and light chain isoforms was used to assay myotube diversification. We found that fused cultures of human satellite cells express adult fast and slow MHCs in addition to the embryonic and fetal isoforms. Only the four fast light chains (MLC1emb, MLC1F, MLC2F and MLC3F) were synthesized. No slow MLCs were ever detected in these cultures. In order to determine if the human satellite cells were committed to distinct fast and slow myogenic lineages, a clonal analysis was carried out on both cell populations. All myogenic clones expressed fast and slow MHCs, suggesting that there is no evidence for different fast and slow satellite cell lineages in human skeletal muscle.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0960-8966(93)90080-4 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The microbial mechanism of maize residue decomposition under different temperature and moisture regimes in a Solonchak.
Sci Rep
January 2025
Shaanxi Province Key Laboratory of Bio-resources, School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, 723000, China.
Soil salinization becomes serious under climate change and human activities. Although the residue decomposition contributes lots to soil carbon storage and fertility, the decomposition process and microbial mechanisms on saline-alkali soils are still vague facing climate change. We measured the mass loss of residue (0, 4, 8, 15, 30, 60 and 90 days), CO emission (every two days), and the microbial community structure (0, 4, 15 and 90 days) by using the litter bag method, gas chromatography and high-throughput sequencing technology during the residue decomposition (90 days) in a saline-alkali soil from the Tarim River Basin, China under various temperatures (15 °C, 25 °C, 35 °C) and soil moisture levels (20%, 40%, 60% water holding capacity).
View Article and Find Full Text PDFWhole-Cell Thermal Sensor for the Detection of -Infected Erythrocytes: Imprinted Polymers as Synthetic Receptors for the Detection of Malaria.
ACS Sens
January 2025
Sensor Engineering Department, Faculty of Science and Engineering, Maastricht University, P.O. Box 616, 6200 MDMaastricht, The Netherlands.
Malaria is a major public healthcare concern worldwide, representing a leading cause of death in specific regions. The gold standard for diagnosis is microscopic analysis, but this requires a laboratory setting, trained staff, and infrastructure and is therefore typically slow and dependent on the experience of the technician. This study introduces, for the first time, a biomimetic sensing platform for the direct detection of the disease.
View Article and Find Full Text PDFPhotodissociation Dynamics of Formic Acid at 230 nm: A Computational Study of the CO and CO Forming Channels.
J Phys Chem A
January 2025
Department of Chemistry, National Chung Hsing University, Taichung 402, Taiwan.
Recent photolysis experiments with formic acid suggest that the roaming mechanism is a significant CO-forming pathway at a photolysis energy of 230 nm. While previous computational studies have identified multiple dissociation pathways for CO-forming channels, the dynamic features of these pathways remain poorly understood. This study investigates the dissociation dynamics of the CO + HO and CO + H channels in the ground state (S) of formic acid using direct dynamics simulation and the generalized multi-center impulsive model (GMCIM) at 230 nm.
View Article and Find Full Text PDFWe report a case of long RP' tachycardia diagnosed as fast-slow atrioventricular nodal reentrant tachycardia (AVNRT) with a bystander nodoventricular pathway (NVP). Differential responses to right ventricular extrastimuli from the base and apex highlighted the anatomical proximity of the NVP attachment, contributing to the diagnosis.
View Article and Find Full Text PDFQuantum ghost imaging (QGI) leverages correlations between entangled photon pairs to reconstruct an image using light that has never physically interacted with an object. Despite extensive research interest, this technique has long been hindered by slow acquisition speeds, due to the use of raster-scanned detectors or the slow response of intensified cameras. Here, we utilize a single-photon-sensitive time-stamping camera to perform QGI at ultra-low-light levels with rapid data acquisition and processing times, achieving high-resolution and high-contrast images in under 1 min.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!