The method has been developed for accounting viable bifidobacteria in the complex preparation biphicol using the growth inhibitor of Escherichia coli (0.01% solution of sodium azide), added to the growing medium. Choice of sodium azide concentration inhibiting growth of Escherichia coli M-17 and not affecting the growth of Bifidobacterium bifidum 1 is described in detail. Data are presented on the quality of commercial preparation made at different enterprises.
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Appl Microbiol Biotechnol
January 2025
College of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng, 224051, PR China.
L-valine holds wide-ranging applications in medicine, food, feed, and various industrial sectors. Escherichia coli, a pivotal strain in industrial L-valine production, features a concise fermentation period and a well-defined genetic background. This study focuses on mismatch repair genes (mutH, mutL, mutS, and recG) and genes associated with mutagenesis (dinB, rpoS, rpoD, and recA), employing a high-glucose adaptive culture in conjunction with metabolic modifications to systematically screen for superior phenotypes.
View Article and Find Full Text PDFACS Appl Mater Interfaces
January 2025
Tianjin Key Laboratory for Marine Environmental Research and Service, School of Marine Science and Technology, Tianjin University, Tianjin 300072, China.
Marine biofouling and corrosion have become the main problems affecting the development of the marine industry. Silicone-based coatings have been widely used for antifouling and anticorrosion due to their low surface energy. However, the poor adhesion and low mechanical stability of these materials limit their application in complex marine environments.
View Article and Find Full Text PDFJ Integr Plant Biol
January 2025
Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, 510631, China.
A synthetic biology approach using a robust reconstitution system in Escherichia coli enables the identification of plant ubiquitin-like proteases responsible for removing the small ubiquitin-like modifier (SUMO) post-translational modifications from specific protein substrates.
View Article and Find Full Text PDFBackground: When haemolytic anaemia, thrombocytopenia and renal failure are present, a thrombotic microangiopathic (TMA) condition should be suspected. We describe the various differential diagnoses of primary TMA syndromes, their clinical findings, clinical workup and treatment.
Case Presentation: A previously healthy man in his fifties was hospitalised with anaemia, thrombocytopenia, bilirubinaemia and acute renal failure.
Biomacromolecules
January 2025
School of Life Science and Health Engineering, Jiangnan University, Wuxi 214122, China.
Three chondroitin sulfate (CS) analogues with predominant subtypes (A, C, and E) were prepared from engineered K4 combined with regioselective sulfation. CS with the designed sulfates as the main components was characterized by nuclear magnetic resonance spectroscopy, elementary analysis, and disaccharide analysis. CS prepared from the native or degraded capsular polysaccharide had molecular weights of 1.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!