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Comparative evaluation of traditional and molecular diagnostic methods for malaria: An analysis of performance.
Trop Parasitol
February 2024
Department of Pathology, Kasturba Medical College, Manipal, Karnataka, India.
Purpose: As we edge closer to the eradication of malaria, several methods for detecting species have been developed, including peripheral blood smear examination (PBS), rapid diagnostic tests (RDTs), serological evaluations, fluorescent microscopy, polymerase chain reactions (PCRs), fluorescent hybridization, and flow cytometry. The suitability of these tools for routine diagnosis requires evaluation, considering both their diagnostic accuracy and cost-effectiveness.
Materials And Methods: Our study compared four diagnostic techniques for malaria: PBS, quantitative buffy coat (QBC), RDT, and PCR.
Complete Blood Count as point of care testing QBC STAR™: Preliminary evaluation.
Int J Lab Hematol
October 2021
Regional Reference Center for the Laboratories Quality, Hospital ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.
Introduction: Point of care testing (POCT) represents a valuable option when laboratory data shall be urgently available for timely clinical management, with a turnaround time (TAT) that is unfeasible using conventional laboratory instrumentation. This study was aimed to compare the performance of QBC STAR™ compared to Sysmex XN-module and the reference optical microscopy (OM) assessment.
Material And Methods: One hundred peripheral blood samples, collected in K EDTA tubes, and 50 capillary blood samples obtained by finger stick were analyzed with QBC STAR™, Sysmex XN-module, and OM.
Comparision of the various routine diagnostic modalities of malaria and a new method: the Parasight™ platform.
J Parasit Dis
September 2020
Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, 2nd Floor, institute Block, JIPMER, Dhanvantri Nagar, Puducherry, 605006 India.
Malaria is one of most important parasitic disease, which is still much prevalent in India. The burden of malaria in India is complex and the proportions of and vary across India, because of the highly variable malaria eco-epidemiological profiles, transmission factors, and the presence of multiple species and vectors. The diagnostic modalities which were being used currently, are at the risk of missing potential malaria cases, if a single test is being used for a given sample.
View Article and Find Full Text PDFPerformance evaluation of different strategies based on microscopy techniques, rapid diagnostic test and molecular loop-mediated isothermal amplification assay for the diagnosis of imported malaria.
Clin Microbiol Infect
January 2020
Department of Parasitology-Mycology, Toulouse University Hospital, Toulouse, France; Center for Pathophysiology Toulouse Purpan (CPTP), University of Toulouse, CNRS, INSERM, UPS, Toulouse, France. Electronic address:
Objectives: Malaria is one of most common tropical diseases encountered in travellers and migrants. It requires an urgent and reliable diagnosis considering its potential severity. In this study, performance of five diagnostic assays were evaluated in a nonendemic region and compared prospectively to quantitative PCR (qPCR).
View Article and Find Full Text PDFMultiplex real-time PCR for diagnosing malaria in a non-endemic setting: a prospective comparison to conventional methods.
Eur J Clin Microbiol Infect Dis
December 2018
Department of Medical Microbiology, Leiden University Medical Center, Albinusdreef 2, 2333 ZA, Leiden, The Netherlands.
Almost a decade ago our diagnostic laboratory implemented an in-house real-time PCR for the detection of Plasmodium DNA to diagnose malaria in parallel with conventional diagnostics, i.e., microscopy (thick and thin smears), quantitative buffy coat microscopy (QBC), and a rapid diagnostic test (RDT).
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