The growth arrest-specific (gas) genes were initially identified on the basis of their preferential expression in mouse fibroblasts during quiescence, followed by down-regulation upon reentry into the cell cycle. We here report studies on the expression of these genes in murine fibroblasts undergoing replicative senescence in vitro. Our results indicate a different behavior between senescent and GO-arrested quiescent fibroblasts. Expression of the gas1 and 6 genes was dramatically reduced in senescent cells. Only basal levels of gas2, 3, and 5 genes were detected in senescent fibroblasts, and they were independent of the growing conditions of the cultures. Down-regulation of the gas1 gene expression in senescent cells was apparently due to reduced transcription of the gas1 gene. This correlates with an altered pattern of factors that bind to the promoter region of the gas1 gene, as measured by band shift assay with nuclear extracts of senescent fibroblasts.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1006/excr.1994.1078 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Identification of signatures associated with microsatellite instability and immune characteristics to predict the prognostic risk of colon cancer.
Open Med (Wars)
December 2024
Department of Immunology, Basic Medical Institute, Chengde Medical College, Chengde 067000, Hebei, China.
Background: Microsatellite instability (MSI) significantly impacts treatment response and outcomes in colon cancer; however, its underlying molecular mechanisms remain unclear. This study aimed to identify prognostic biomarkers by comparing MSI and microsatellite stability (MSS).
Methods: Data from the GSE39582 dataset downloaded from the Gene Expression Omnibus database were analyzed for differentially expressed genes (DEGs) and immune cell infiltration between MSI and MSS.
Hedgehog-dependent and hedgehog-independent roles for growth arrest specific 1 in mammalian kidney morphogenesis.
Development
December 2024
Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
Growth arrest specific 1 (GAS1) is a key regulator of mammalian embryogenesis, best known for its role in hedgehog (HH) signaling, but with additional described roles in the FGF, RET, and NOTCH pathways. Previous work indicated a later role for GAS1 in kidney development through FGF pathway modulation. Here, we demonstrate that GAS1 is essential for both mesonephrogenesis and metanephrogenesis - most notably, Gas1 deletion in mice results in renal agenesis in a genetic background-dependent fashion.
View Article and Find Full Text PDFIntegrated multiomics characterization reveals cuproptosis-related hub genes for predicting the prognosis and clinical efficacy of ovarian cancer.
Front Immunol
November 2024
Department of Gynecologic Oncology, Zhejiang Cancer Hospital, Hangzhou, China.
Article Synopsis
- Ovarian cancer (OC) is a serious health issue for women, characterized by a poor prognosis and limited treatment options, requiring further study on its underlying mechanisms and immune interactions.
- The research focused on identifying cuproptosis-related genes (CRGs) to categorize OC into distinct subtypes and create a risk score (RS) model for predicting patient outcomes using data from various databases.
- The findings established three clinical subtypes of OC based on CRG expression, highlighted six key hub genes with oncogenic properties, and emphasized the potential of the RS model to improve survival predictions and therapeutic strategies.
You Don't Always Get What You Want!
Anesthesiology
October 2024
Department of Anesthesiology and Pain Medicine, University of Washington and Seattle Children's Research Institute, Seattle, Washington.
Background: Mutations in several genes of Caenorhabditis elegans confer altered sensitivities to volatile anesthetics. A mutation in one gene, gas-1(fc21), causes animals to be immobilized at lower concentrations of all volatile anesthetics than in the wild type, and it does not depend on mutations in other genes to control anesthetic sensitivity. gas-1 confers different sensitivities to stereoisomers of isoflurane, and thus may be a direct target for volatile anesthetics.
View Article and Find Full Text PDFPreexisting senescent fibroblasts in the aged bladder create a tumor-permissive niche through CXCL12 secretion.
Nat Aging
November 2024
Division of Cancer Cell Biology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
Article Synopsis
- * These p16-sn fibroblasts accumulate with age, act as inflammatory cancer-associated fibroblasts (CAFs), and significantly contribute to bladder tumor growth by expressing the CXCL12 gene.
- * Targeting p16-sn cells or blocking CXCL12 signaling can reduce bladder tumor growth, indicating that these cells create an environment that supports cancer development in older individuals.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!