The anti-proliferative effects of nicotinamide and 3-aminobenzamide on human smooth muscle cells in vitro.

The proliferation of cultured human arterial smooth muscle cells (HASMC) was suppressed by nicotinamide and its analogue, 3-aminobenzamide, in a concentration-dependent manner, based on cell count and [3H]thymidine incorporation into DNA. The addition of 10 and 15 mM nicotinamide, or 6 mM 3-aminobenzamide, to HASMC, for 96 h, resulted in a 19.3%, 44% and 41.5% reduction of cell growth and inhibition of DNA synthesis. Since c-myc protooncogene expression has previously been correlated with cell proliferation, the steady state level of its mRNA was determined in control and treated HSMC by Northern analysis, following a 1 h treatment with the respective chemicals. Whereas c-myc mRNA was suppressed by nicotinamide in proportion to its ability to reduce HASMC growth, down regulation of c-myc by 3-aminobenzamide was much less than the observed antiproliferative effect. These results suggest that the growth regulatory properties of nicotinamide and 3-aminobenzamide in HASMC are probably unrelated to their activity in controlling c-myc gene expression.