Purpose: To investigate variations in diabetic short-wavelength sensitivity with acute, induced changes in blood glucose level.

Methods: Increment threshold measures were obtained for short-wavelength-sensitive and middle/long-wavelength-sensitive cone pathways after an induced, acute change in blood glucose level in diabetic observers.

Results: Diabetic observers showed an increase in short-wavelength sensitivity, but no change in middle/long-wavelength sensitivity, with a rapid drop in blood glucose level.

Conclusions: Experimentally induced changes in diabetic blood glucose levels can directly affect diabetic short-wavelength sensitivity.

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