It has been shown that PDGF plays a role in the regulation of lens growth and differentiation. PDGF occurs in vivo as a homodimer or heterodimer of the two polypeptide chains A and B. These isoforms bind with different affinities to two distinct receptor types, termed alpha and beta. In order to identify the different PDGF receptors on cultured bovine lens epithelial cells (BLEC), we performed receptor binding studies, using 125I-labelled PDGF isoforms. Analysis of our data revealed that BLEC expressed approximately 35,000 PDGF-BB binding sites (KD = 21 ng/ml) and 9000 PDGF-AB binding sites (KD = 11 ng/ml), but only 4800 PDGF-AA binding sites (KD = 7 ng/ml). This study represents the first demonstration that lens epithelial cells express PDGF receptors.
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