The Photinus pyralis and Luciola mingrelica luciferases genes expression kinetics has been studied, repectively, on the pME61 and pJG lambda plasmids with a thermoinducible lambda PR promoter in the E. coli strain CA under conditions of a pH shift. An increase in the enzyme activity after a pH shift has been revealed. The maximal amount of the firefly Ph. pyralis luciferase reaches 5.3%, while the maximal amount of the L. mingrelica enzyme reaches 4.9% of the total amount of cellular proteins in case of pHout shift to 9.0 according to the SDS gel electrophoresis data; that in case of a pHout shift to 8.5 is 4.9% and 4.5%, respectively. The enzyme activation correlates well with the dynamics of pHout and pHin.

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