By means of successive GL-affinity and Mono S column chromatographies (HPLC), a 100 kDa GL-binding protein (gp100) was purified from the partially purified CK-II fraction of EAT cells as an effective phosphate acceptor for CK-II. It was found that (i) gp100 (pI 9.0) is copurified with CK-II, Hsp-90 and p34 or p70; (ii) gp100 cross-reacts with anti-human GR; (iii) phosphorylation of gp100 by CK-II is significantly stimulated by 1 microM GL or 0.3 microM oGA; and (iv) GL as well as DEX inhibit it at doses above 3 microM. Data are provided to suggest that the GL-induced selective inhibition of the CK-II catalyzed phosphorylation of gp100 may be involved in the anti-inflammatory effects of GL.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1006/bbrc.1994.1155 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Microsomal glutathione transferase 1 controls metastasis and therapeutic response in melanoma.
Pharmacol Res
October 2023
Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston, SC 29425, United States. Electronic address:
Article Synopsis
- - Recent therapies for malignant melanoma show promise, but most patients develop resistance, highlighting the need for new drug targets focused on redox homeostasis and lipid peroxidase pathways to improve treatment outcomes.
- - Researchers found that microsomal glutathione S-transferase 1 (MGST1), a specific enzyme, is highly expressed in drug-resistant melanomas and plays a crucial role in tumor spread and response to therapy.
- - Reducing MGST1 levels in melanoma cells increased oxidative stress and made them more susceptible to immune attack and anticancer drugs, leading to reduced metastasis and improved survival in mouse models.
Syntenin regulates melanogenesis via the p38 MAPK pathway.
Mol Med Rep
August 2020
Central Laboratory of Shaanxi Provincial People's Hospital, Xi'an, Shaanxi 710068, P.R. China.
Melanogenesis is the synthesis of the skin pigment melanin, which serves a critical role in the study of pigmentary skin diseases. Syntenin has been identified as a melanosome protein, but its role in melanogenesis is not completely understood. The present study aimed to investigate the effects and mechanisms underlying syntenin on melanogenesis in immortalized human melanocytes.
View Article and Find Full Text PDFPLX4032 Mediated Melanoma Associated Antigen Potentiation in Patient Derived Primary Melanoma Cells.
J Cancer
December 2015
1. New York Medical College, Department of Microbiology and Immunology, Valhalla, New York, 10595;
Over expression of various immunogenic melanoma associated antigens (MAAs) has been exploited in the development of immunotherapeutic melanoma vaccines. Expression of MAAs such as MART-1 and gp100 is modulated by the MAPK signaling pathway, which is often deregulated in melanoma. The protein BRAF, a member of the MAPK pathway, is mutated in over 60% of melanomas providing an opportunity for the identification and approval by the FDA of a small molecule MAPK signaling inhibitor PLX4032 that functions to inactivate mutant BRAF(V600E).
View Article and Find Full Text PDFNobiletin, a polymethoxy flavonoid, reduced endothelin-1 plus SCF-induced pigmentation in human melanocytes.
Photochem Photobiol
September 2015
Research Institute for Biological Functions, Chubu University, Kasugai, Aichi, Japan.
Nobiletin is a unique flavonoid having polymethoxy groups and has exhibited anti-inflammatory and antiobesity effects. Here, we examined the inhibition of nobiletin on melanogenesis induced by endothelin-1 (ET) and stem cell factor (SCF) in normal human melanocytes. Nobiletin dose dependently reduced ET plus SCF-stimulated tyrosinase activity without causing cytotoxicity.
View Article and Find Full Text PDFThe peripheral clock regulates human pigmentation.
J Invest Dermatol
April 2015
The Centre for Dermatology Research, Institute of Inflammation and Repair, University of Manchester, Manchester, UK; Department of Dermatology, University of Muenster, Muenster, Germany. Electronic address:
Although the regulation of pigmentation is well characterized, it remains unclear whether cell-autonomous controls regulate the cyclic on-off switching of pigmentation in the hair follicle (HF). As human HFs and epidermal melanocytes express clock genes and proteins, and given that core clock genes (PER1, BMAL1) modulate human HF cycling, we investigated whether peripheral clock activity influences human HF pigmentation. We found that silencing BMAL1 or PER1 in human HFs increased HF melanin content.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!