A simple fluorimetric method for the estimation of ligand binding parameters in ligand-enzyme complexes.

The theory and practice of a simple fluorimetric method for the estimation of ligand binding parameters in ligand-enzyme complexes is described. In this method, the concentration of a ligand-binding enzyme and the dissociation constant of a ligand-enzyme complex were estimated solely from the total concentration of a ligand and the total fluorescence of the ligand in the absence and in the presence of enzyme.