1. Increased levels of bone alkaline phosphatase activity were observed in diabetic rats. These animals exhibited impaired bone development without concomitant alterations of the sequence of cellular transformations. 2. Alkaline phosphatase activity was delayed in diabetic rats but the kinetic parameters for the hydrolysis of p-Nitrophenylphosphate (PNPP) were virtually the same observed for controls (N = 1.2 and K0.5 = 43 microM). 3. Alkaline phosphatase from diabetic rats had a better affinity (K0.5 = 38 microM) for magnesium ions than controls (K0.5 = 91 microM). 4. Zinc ions affected alkaline phosphatase activity from control and diabetic rats in the same way (K0.5 = 10 microM).
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0300-9629(93)90448-d | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Glycan-Matchmade Multivalent Decoration of Enzyme Labels for Amplified Electrochemical Detection of Glycoproteins.
Anal Chem
January 2025
Center for Advanced Analytical Science, Guangzhou Key Laboratory of Sensing Materials and Devices, Guangdong Engineering Technology Research Center for Sensing Materials and Devices, School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou 510006, China.
Glycoproteins are of significant value to liquid biopsy of human diseases. Herein, we present a universal electrochemical platform for the amplified detection of glycoproteins, taking advantage of the glycan-matchmade multivalent decoration of enzyme labels for the enzymatic signal amplification. Briefly, the glycan-matchmade multivalent decoration involves two steps, i.
View Article and Find Full Text PDFHeat stress promotes osteogenic and odontogenic differentiation of stem cells from apical papilla via glucose-regulated protein 78-mediated autophagy.
J Dent Sci
January 2025
Endodontic Department, Changzhou Stomatological Hospital, Changzhou, China.
Background/purpose: Heat stress is essential for improving the efficacy of mesenchymal stem cell (MSC)-based regeneration medicine. However, it is still unclear whether and how heat stress influences the differentiation of stem cells from apical papilla (SCAPs). This research aimed to explore the potential mechanism of glucose-regulated protein 78 (GRP78) in regulating differentiation under heat stress in SCAPs.
View Article and Find Full Text PDFOptimized human dedifferentiated fat cells from the buccal fat pad-derived osteoinductive extracellular vesicles promote osteoblast differentiation.
J Dent Sci
January 2025
Second Department of Oral and Maxillofacial Surgery, Osaka Dental University, Osaka, Japan.
Background/purpose: Bone reconstruction in the maxillofacial region typically relies on autologous bone grafting, which presents challenges, including donor site complications and graft limitations. Recent advances in tissue engineering have identified highly pure and proliferative dedifferentiated fat cells (DFATs) as promising alternatives. Herein, we explored the capacity for osteoblast differentiation and the osteoinductive characteristics of extracellular vesicles derived from DFATs (DFAT-EVs).
View Article and Find Full Text PDFEnhancement of titanium surfaces using different acid solutions at room temperature to improve bone cell responses.
J Dent Sci
January 2025
School of Dental Technology, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.
Background/purpose: Early osseointegration of titanium (Ti) dental implants relies on the surface topography. Surface modification of Ti seeks to enhance bone regeneration around implants. Acid etching is the simple, less technique sensitive and cost-effective technique for surface treatment.
View Article and Find Full Text PDFBeta-adrenergic receptor antagonist propranolol prevents bisphosphonate-related osteonecrosis of the jaw by promoting osteogenesis.
J Dent Sci
January 2025
State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Background/purpose: Bisphosphonate-related osteonecrosis of the jaw (BRONJ), a complication arising from the use of bisphosphonates (BPs), inflicts long-term suffering on patients. Currently, there is still a lack of effective treatments. This study aimed to explore the preventive effects of propranolol (PRO) on BRONJ in vitro and in vivo, given PRO's potential in bone health enhancement.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!