Deoxyribonuclease activity in Echinococcus granulosus protoscoleces and hydatid fluid.

Int J Parasitol

Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Buenos Aires, Argentina.

Published: July 1994

Deoxyribonuclease activity was detected in E. granulosus protoscoleces from sheep hydatid cysts by electrophoresis in agarose gels of DNA fragments obtained after incubation of integral DNA with a protoscoleces preparation. Preliminary characterization showed that deoxyribonuclease activity was optimal at neutral-alkaline pH, magnesium ions were required, and it was able to digest different types of DNA, making random cuts. Electrophoresis in DNA-containing sodium dodecylsulfate (SDS)-polyacrylamide gels indicated a relative molecular mass, under non-reducing conditions, of 32 kDa. Deoxyribonuclease activity was also found in sheep hydatid fluid. It shared optimal pH, ionic and substrate requirements with the enzyme from protoscoleces but had a higher relative molecular mass (40 kDa), the same as that of normal sheep serum deoxyribonuclease.

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http://dx.doi.org/10.1016/0020-7519(94)90153-8DOI Listing

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