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Highly-multiplex detection of plasma cell-free human papillomavirus-16 DNA in oropharyngeal carcinoma.
J Clin Virol
January 2025
Center for Immunotherapy and Precision Immuno-Oncology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA; Department of Radiation Oncology, Taussig Cancer Institute, Cleveland Clinic, Cleveland, OH, USA. Electronic address:
Background: Plasma cell-free Human Papillomavirus DNA (cfHPVDNA) is a biomarker for oropharyngeal carcinoma. Existing diagnostics may be limited by inadequate sensitivity or high cost/complexity for longitudinal monitoring.
Objectives: We hypothesized that sensitive and specific plasma cfHPVDNA detection may be achieved via a highly-multiplex qPCR method.
Multiplex one-step direct asymmetric PCR of blood and dual-labelled probe-mediated melting curve for genotyping of MTHFR and MTRR polymorphisms.
RSC Adv
January 2025
Nanobiosensing and Microfluidic Point-of-Care Testing, Key Laboratory of Luzhou, Department of Clinical Laboratory, The Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University Luzhou Sichuan 646000 PR China
Accurate, rapid, and multiplex SNP analysis holds significant clinical value. However, the inevitable nucleic acid extraction, involving centrifugation, heating, and magnetic separation, is often time-consuming. In this study, direct blood PCR was combined with dual-labelled probe-mediated melting curves to identify SNPs corresponding to MTHFR (C677T, rs#1801133 and A1298C, rs#1801131) and MTRR (A66G, rs#1801394) in a single tube.
View Article and Find Full Text PDFA Multiplex High-Resolution Melting (HRM) assay to differentiate Fusarium graminearum chemotypes.
Sci Rep
December 2024
Cereal Disease Laboratory, Agricultural Research Service, US Department of Agriculture, St. Paul, MN, 55108, USA.
Fusarium graminearum is a primary cause of Fusarium head blight (FHB) on wheat and barley. The fungus produces trichothecene mycotoxins that render grain unsuitable for food, feed, or malt. Isolates of F.
View Article and Find Full Text PDFA chromosome-scale genome assembly of mungbean ().
PeerJ
December 2024
National Omics Center, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, Thailand.
Background: Mungbean () is one of the most socio-economically important leguminous food crops of Asia and a rich source of dietary protein and micronutrients. Understanding its genetic makeup is crucial for genetic improvement and cultivar development.
Methods: In this study, we combined single-tube long-fragment reads (stLFR) sequencing technology with high-throughput chromosome conformation capture (Hi-C) technique to obtain a chromosome-level assembly of cultivar 'KUML4'.
Open-closed single-tube on-beam tuning-fork-enhanced fiber-optic photoacoustic spectroscopy.
Photoacoustics
October 2024
Wuhan National Laboratory for Optoelectronics (WNLO) and National Engineering Research Center of Next Generation Internet Access-system, School of Optical and Electronic Information, Huazhong University of Science and Technology, Wuhan 430074, China.
A proof-of-concept on-beam tuning-fork-enhanced photoacoustic sensor based on an open-closed single-tube acoustic-microresonator (AmR) was proposed and investigated for the first time, to the best of our knowledge. Due to the high acoustic amplification effect, the open-closed AmR improved the detection sensitivity by 54 times with respect to the bare tuning fork (TF). Compared to traditional dual-tube/single-tube on-beam spectrophone configuration, the developed approach significantly facilitates the laser beam alignment and reduces the sensor size and gas consumption.
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