Using the indirect immunofluorescence technique in presence of anti-adenovirus-5 antibody, fluorescent spots were observed two hours after adsorption at 4 degrees C onto HeLa cells on the surface of the cells. The spots subsequently became intracytoplasmic and persisted about 7 to 8 h. Intense fluorescence was further observed in the whole cytoplasm and in the nucleus 12 h following the injection.

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