The inhalation toxicity of a commercial proteolytic enzyme preparation containing 12% subtilisin Carlsberg was studied in experimental animals. Guinea pigs that had been pretreated by a series of intradermal injections of the enzyme preparation in saline solution died as a result of a single 6-hour exposure to the enzyme preparation at an air concentraion of 41.2 mg/m-3, while normal guinea pigs and pretreated guinea pigs that were dosed with an antihistamine immediately prior to exposure survived. The 6-hour LC50 for pretreated guinea pigs was determined as 24.7 mg/m-3. Normal rats, normal rabbits, and pretreated rabbits survived exposures to the enzyme preparation at concentrations as high as 36.8 mg/m-3. Pathologic examinations revealed changes only in the lungs of the exposed animals. These pulmonary alterations appear to be reversible. A histamine release is suggested as the primary effect of a secondary exposure to this proteolytic enzyme preparation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/0002889758507246 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effect of Elevated Temperature on Compressive Strength of MICCP and EICCP Biocemented Mortar.
Mol Biotechnol
January 2025
Department of Civil Engineering, Visvesvaraya National Institute of Technology (VNIT), Nagpur, Maharashtra, 440010, India.
Recently biocementation has got attention of many researchers worldwide as one of the most potent techniques for sustainable construction. Several studies have been carried out worldwide on biocementation by urea hydrolysis. Biocementation by bacterially induced calcium carbonate precipitation by different bacterial species has been among the most widely researched areas in this field.
View Article and Find Full Text PDFSurface immobilization of single atoms on heteroatom-doped carbon nanospheres through phenolic-mediated interfacial anchoring for highly efficient biocatalysis.
Chem Sci
January 2025
BMI Center for Biomass Materials and Nanointerfaces, National Engineering Laboratory for Clean Technology of Leather Manufacture, Ministry of Education Key Laboratory of Leather Chemistry and Engineering, College of Biomass Science and Engineering, Sichuan University Chengdu Sichuan 610065 China
Single-atom catalysts (SACs) dispersed on support materials exhibit exceptional catalytic properties that can be fine-tuned through interactions between the single atoms and the support. However, selectively controlling the spatial location of single metal atoms while simultaneously harmonizing their coordination environment remains a significant challenge. Here, we present a phenolic-mediated interfacial anchoring (PIA) strategy to prepare SACs with Fe single atoms anchored on the surface of heteroatom-doped carbon nanospheres.
View Article and Find Full Text PDFFlower-like Biomimetic Enzyme for Rapid and Sensitive Detection of Zearalenone in Vegetable Oil Deodorizer Distillate.
Anal Biochem
January 2025
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address:
In order to achieve high quality production of vitamin E and plant sterols, it is necessary to conduct rapid and accurate detection of fungal toxins in their production raw material (vegetable oil deodorizer distillate, VODD). In this study, the flower-like biomimetic enzyme of silver-doped ZnO was synthesized through wet chemical method and in-situ reduction method. Based on above work, a flower-like biomimetic enzyme modified glass carbon electrode was fabricated, and its excellent detection capability against fungal toxins zearalenone was confirmed through electrochemical analysis.
View Article and Find Full Text PDFHollow mesoporous silica nanoparticles for drug formulation and delivery: Opportunities for cancer therapy.
Colloids Surf B Biointerfaces
January 2025
Shaanxi Key Laboratory of Catalysis, School of Chemistry and Environment Science, Shaanxi University of Technology, No.1 East 1st Ring Road, Hanzhong, Shaanxi 723001, PR China.
The advantages of large surface area, high volume ratio, good biocompatibility, and controllable surface functionalization make hollow mesoporous silica nanoparticles (HMSNs) an ideal drug carrier. HMSNs can achieve high efficiency, targeting, and controlled release by adjusting the microstructure and surface modification of its particles, which makes it broad application prospects in the field of medical therapy, especially in cancer therapy. Numerous studies have shown that preparation method, shape, particle size, hollow inner diameter, aperture and wall thickness of the HMSNs, the characteristics of the drugs, the interaction between the drugs and the carriers, and the external environment all closely affect the drug delivery, release, and efficacy.
View Article and Find Full Text PDFNear-Native Imaging of Metal Ion-Initiated Cell State Transition.
ACS Nano
January 2025
National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei 230026, China.
Metal ions are indispensable to life, as they can serve as essential enzyme cofactors to drive fundamental biochemical reactions, yet paradoxically, excess is highly toxic. Higher-order cells have evolved functionally distinct organelles that separate and coordinate sophisticated biochemical processes to maintain cellular homeostasis upon metal ion stimuli. Here, we uncover the remodeling of subcellular architecture and organellar interactome in yeast initiated by several metal ion stimulations, relying on near-native three-dimensional imaging, cryo-soft X-ray tomography.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!