We present two techniques for determining the concentration and 13C enrichment of acetate in biological fluids. After the sample has been spiked with an internal standard of [2,2,2,2H3,1-13C]acetate, acetate is first enzymatically converted to acetyl-coenzyme A, which is chemically converted to acetylglycine. The latter is analyzed by gas chromatography-mass spectrometry, either as a methyl ester by positive chemical ionization or as a pentafluorobenzyl ester by negative chemical ionization. The mole percentage enrichment of tissue acetyl-CoA can also be assayed after conversion to acetylglycine pentafluorobenzyl ester.
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