The effects of tri-n-butyltin chloride (TBT) on ionic homeostasis on isolated trout hepatocytes were investigated by flow cytometry (FCM), using the Ca(2+)-sensitive and pH-sensitive fluorescent probes Indo-1 and SNARF-1, respectively. Cell viability was monitored concurrently. Treatment of hepatocytes with 1 and 5 microM TBT caused a rapid and sustained elevation of cytosolic free Ca2+ concentration [Ca2+]i and an important cytoplasmic acidification. These changes were dependent upon TBT concentration and were maintained over 60 min, the maximum exposure period investigated. At 0.5 microM TBT, there was a slight but not significant increase in [Ca2+]i and a significant reduction in intracellular pH (pHi) only after 60 min of exposure. A rise in [Ca2+]i and cytoplasmic acidification were observed before loss of viability was detectable. Experiments carried out in Ca(2+)-free medium suggest that TBT mainly mobilizes Ca2+ from intracellular stores in trout hepatocytes. The cytoplasmic acidification following TBT exposure seems to be caused by the combination of intracellular Ca2+ mobilization and by direct action of TBT. The present results suggest that ionic homeostasis perturbations could be early events in the mechanism of cell injury by TBT.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1006/abbi.1994.1326 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Protective Effects of 17-βE on the Primary Hepatocytes of Rainbow Trout () Under Acute Heat Stress.
Antioxidants (Basel)
October 2024
Department of College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
The rainbow trout () is a typical cold-water species. However, due to global warming, it has experienced prolonged high-temperature stress. Research indicates that thermotolerance in rainbow trout varies by sex at multiple physiological levels.
View Article and Find Full Text PDFComparative analysis of 3D and 2D in vitro models of the permanent fish liver cell line RTL-W1: Metabolic capabilities and responses to xenobiotics.
Ecotoxicol Environ Saf
December 2024
Department of Biological and Environmental Sciences, University of Gothenburg, Gothenburg 405 30, Sweden. Electronic address:
In vitro models based on permanent fish liver cell lines have proven to be versatile tools for examining chemical biotransformation and toxicity. However, their in vivo relevance remains uncertain due to their potentially de-differentiated phenotype. Here, we investigate whether a 3D cell culture environment can restore hepatocyte-like properties of the Rainbow trout liver cell line RTL-W1.
View Article and Find Full Text PDFFibroblast growth factor 1 (FGF1) improves glucose homeostasis, modulates gut microbial composition, and reduces inflammatory responses in rainbow trout (Oncorhynchus mykiss) fed a high-fat diet.
Int J Biol Macromol
November 2024
Northwest A&F University, China. Electronic address:
Article Synopsis
- * A study tested a special protein called rFGF1 on rainbow trout to see if it could help fix these problems caused by the high-fat diet.
- * The results showed that rFGF1 lowered sugar levels and improved liver health without causing extra problems, and it also changed the good bacteria in the fish’s guts, which might help with their health.
Environmentally Relevant Concentrations of -6PPD-Quinone Caused More Serious Hepatotoxicity Than -Enantiomer and Racemate in .
Environ Sci Technol
October 2024
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products/Key Laboratory of Detection for Pesticide Residues and Control of Zhejiang, Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, P. R. China.
6PPD-quinone (6PPD-Q) is frequently detected in various environmental media, and the environmentally relevant concentrations can be fatal to . Notably, 6PPD-Q has two enantiomers (-6PPD-Q and -6PPD-Q). In this study, was separately exposed to each enantiomer and racemate of 6PPD-Q for 96 h at environmentally relevant concentrations, and livers were collected.
View Article and Find Full Text PDFEstrogenic Responsiveness of Brown Trout Primary Hepatocyte Spheroids to Environmental Levels of 17α-Ethinylestradiol.
J Xenobiot
August 2024
Team of Animal Morphology and Toxicology, Interdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR), University of Porto (U.Porto), Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos s/n, 4450-208 Matosinhos, Portugal.
Three-dimensional (3D) fish hepatocyte cultures are promising alternative models for replicating in vivo data. Few studies have attempted to characterise the structure and function of fish 3D liver models and illustrate their applicability. This study aimed to further characterise a previously established spheroid model obtained from juvenile brown trout () primary hepatocytes under estrogenic stimulation.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!