The aims of this study were to evaluate the level of mutans streptococci in two groups of Thai (Bangkok) children; to relate the findings to caries prevalence and to the caries increment over 2 years, and to study whether different sealant and fluoride programmes affected levels of mutans streptococci over a 2-year period. The baseline survey comprised 1,114 children aged 12 years. For the sealant project, a minimum of three caries-free permanent molars was required; 752 children aged 12-13 and 512 children aged 7-8 years were distributed into five groups: control group, Delton fissure sealant group, glass ionomer fissure sealant applied by dentist (GIC-dentist group) or by school teachers given a 3-day course (GIC-teacher group), and an HF group (0.5% HF solution applied 3 times). The WHO standard criteria were used to record caries. Prevalence of mutans streptococci was estimated using the Strip mutans test. Mean DMFT (n = 1,114) of 12-years-olds was 2.96; 17% had Strip mutans class 0 (low level), 32% class 1, 33% class 2 and 18% class 3 (the corresponding mean DFT +/- SD for each mutans streptococci class was 1.84 +/- 2.33, 2.23 +/- 2.14, 3.18 +/- 2.75, and 3.59 +/- 3.01 respectively). For the 7- to 8-year-olds (n = 512), mean df teeth at baseline was 5.36 (d = 5.19; f = 0.17); 5% had Strip mutans class 0, 17% class 1, 33% class 2 and 45% class 3 (the corresponding mean df teeth was 3.19 +/- 2.5, 4.13 +/- 2.84, 4.89 +/- 2.94 and 6.39 +/- 3.16 respectively).(ABSTRACT TRUNCATED AT 250 WORDS)

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