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Analyzing Pathophysiology and Immune Cells and Their Cytokines and Mediators in Precision-Cut Slices of the Murine Lung.
Curr Protoc
January 2025
Department of Molecular Pneumology, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, Universitätsklinikum Erlangen, Erlangen, Germany.
Understanding the dynamic pathophysiology of diseases in the lung, such as asthma and chronic asthma, chronic obstructive pulmonary disease, and lung cancer, is crucial for the treatment, analysis, and outcome of these diseases. Unlike other traditional models, we suggest a protocol that is sustainable and reproducible and offers different analysis methods while maintaining in vivo lung architecture and immune dynamics. This protocol allows one to study the pathophysiological changes, including changes to the immune cells, cytokines, and mediators, in 30 precision-cut lung slices from a single murine lung.
View Article and Find Full Text PDFReference values for the Teller Acuity Cards II (TAC II) in infants and preverbal children, a meta-analysis.
Acta Ophthalmol
January 2025
Bartiméus Diagnostic Center for Complex Visual Disorders, Zeist, The Netherlands.
Purpose: The Teller Acuity Card (TAC) procedure is a preferential-looking method to assess visual acuity in infants and preverbal children and provides a quantitative measure of grating acuity. Several studies containing reference values have been published, the majority based on an older version of the TAC card set. In 2003, a new version of the TAC was released, called the TAC II.
View Article and Find Full Text PDFImproving osteogenic properties of zirconia ceramic via glow discharge plasma-enhanced deposition of amine organic compound.
J Dent Sci
January 2025
School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.
Background/purpose: Osseointegration potential is greatly depended on the interaction between bone cells and dental implant surface. Since zirconia ceramic has a bioinert surface, functionalization of the surface with an organic compound allylamine was conducted to overcome its drawback of minimal interaction with the surrounding bone.
Materials And Methods: The zirconia surface was initially treated with argon glow discharge plasma (GDP), then combined with amine plasma at three different conditions of 50-W, 75-W and 85-W, to prepare the final samples.
Membrane-free stem cell components suppress osteoclast differentiation: Implications for oral regenerative treatment.
J Dent Sci
January 2025
Department of Dentistry, Yeungnam University College of Medicine, Daegu, Republic of Korea.
Background/purpose: Membrane-free stem cell components (MFSCCs) have been developed by removing cell membranes with antigens to overcome the limitations associated with cell-based therapies and isolate effective peptides. MFSCCs have been reported to have effects on oral infection sites. Chronic inflammatory diseases cause excessive bone resorption.
View Article and Find Full Text PDFA culture model for the assessment of phenylalanine neurotoxicity in phenylketonuria.
In Vitro Model
February 2022
Institute of Cell Biology and Neurobiology, Charité Anatomy, Charité Universitätsmedizin Berlin, Charitéplatz 1 (intern: Virchowweg 6 CCO), 10117 Berlin, Germany.
Objective: Phenylketonuria (PKU) is caused by a specific mutation of the phenylalanine hydroxylase (PAH) gene. The deficiency of PAH results in high phenylalanine levels (Phe), low tyrosine levels (Tyr), and reduced catecholamine neurotransmitters. The majority of PKU patients, if untreated, develop severe mental retardation.
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