Objective: To examine the relationship between alpha 1-antitrypsin (alpha 1AT) specific activity and tumor necrosis factor alpha (TNF alpha) concentration in synovial fluid from 48 patients with rheumatoid arthritis.
Methods: The specific activity of alpha 1AT was calculated from the measurement of alpha 1AT concentration (by rocket immunoelectrophoresis) and elastase inhibitory capacity. TNF alpha was detected by enzyme-linked immunosorbent assay.
Results: TNF alpha concentrations correlated with the extent of alpha 1AT inactivation.
Conclusion: Our findings are consistent with a role of elastase in TNF alpha release within the inflamed joint.
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| http://dx.doi.org/10.1002/art.1780371203 | DOI Listing |
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