The serum level of rat alpha 1-acid glycoprotein is significantly increased by treatment with phenobarbital, and in vivo studies have shown that phenobarbital seems to act mainly at the transcriptional level. To show the direct mediating effect of phenobarbital on alpha 1-acid glycoprotein gene expression, we investigated the ability of primary cultured rat hepatocytes to respond to in vitro phenobarbital administration. Phenobarbital increased both alpha 1 acid glycoprotein secretion and corresponding mRNA levels in primary rat hepatocytes cultured on matrigel. Used in combination with interleukin-1, interleukin-6 and dexamethasone, phenobarbital had an additive or synergistic effect on alpha 1-acid glycoprotein synthesis. These results show that (a) phenobarbital acts directly on hepatocytes by increasing alpha 1-acid glycoprotein gene expression and (b) this effect is mediated by a specific mechanism independent of pathways involved in alpha 1-acid glycoprotein induction by interleukin-1, interleukin-6 and glucocorticoids.
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Membranes (Basel)
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Nephrology and Renal Transplantation, Hospital Clínic de Barcelona, 08036 Barcelona, Spain.
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Research Center for Care and Control of Infectious Disease, Universitas Padjadjaran, Bandung 45363, Indonesia.
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View Article and Find Full Text PDFDiagnostics (Basel)
December 2024
Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi 371-8511, Japan.
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View Article and Find Full Text PDFBJS Open
December 2024
Institute of Cardiovascular Sciences, University College London, London, UK.
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