Proteins were synthesized in the cell-free coupled transcription-translation systen (S30) under the control of the DNA-templates of plasmid ColEl, phages T2 and Sd. The most active templates were phage T2 DNA and closed plasmid DNA. Plasmid DNA induced synthesis of 8 individual proteins, one of which was a biologically active antibiotic of protein nature--colicin. The titre of colicin synthesized in such a cell-free system reached 1024 U/ml, this being about 100 times greater than the titre of this antibiotic in the colicinogenic bacteria cultures.
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