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Peptide nucleic acid (PNA) clamps reduce amplification of host chloroplast and mitochondria rRNA gene sequences and increase detected diversity in 16S rRNA gene profiling analysis of oak-associated microbiota.
Environ Microbiome
January 2025
School of Natural Sciences, Bangor University, Bangor, UK.
Background: Acquiring representative bacterial 16S rRNA gene community profiles in plant microbiome studies can be challenging due to the excessive co-amplification of host chloroplast and mitochondrial rRNA gene sequences that reduce counts of plant-associated bacterial sequences. Peptide Nucleic Acid (PNA) clamps prevent this by blocking PCR primer binding or binding within the amplified region of non-target DNA to stop the function of DNA polymerase. Here, we applied a universal chloroplast (p)PNA clamp and a newly designed mitochondria (m)PNA clamp to minimise host chloroplast and mitochondria amplification in 16S rRNA gene amplicon profiles of leaf, bark and root tissue of two oak species (Quercus robur and Q.
View Article and Find Full Text PDFRetraction Note: Establishment of an Acanthamoeba keratitis mouse model confirmed by amoebic DNA amplification.
Sci Rep
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Department of Microbiology, Ajou University School of Medicine, Suwon, 16499, Republic of Korea.
An open-label, phase IB/II study of abemaciclib with paclitaxel for tumors with CDK4/6 pathway genomic alterations.
ESMO Open
January 2025
Department of Internal Medicine, Division of Medical Oncology, Yonsei University College of Medicine, Seoul, Republic of Korea. Electronic address:
Background: Disruption of cyclin D-dependent kinases (CDKs), particularly CDK4/6, drives cancer cell proliferation via abnormal protein phosphorylation. This open-label, single-arm, phase Ib/II trial evaluated the efficacy of the CDK4/6 inhibitor, abemaciclib, combined with paclitaxel against CDK4/6-activated tumors.
Patients And Methods: Patients with locally advanced or metastatic solid tumors with CDK4/6 pathway aberrations were included.
First report of causing black foot on walnut in Chile.
Plant Dis
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Universidad de Chile, Departamento de Sanidad Vegetal, Facultad de Ciencias Agronomicas, Casilla 1004, Santiago, Chile, 8820000;
Walnut (Juglans regia L.) is the primary nut tree cultivated in Chile, covering 44.626 ha.
View Article and Find Full Text PDFFirst Report of Causing Black Leaf Spot on in China.
Plant Dis
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Zhejiang Academy of Agricultural Sciences, Institute of Agro-product Safety and Nutrition, Hangzhou, Zhejiang, China;
Chinese yam ( Turcz.), known for its nutrient-rich underground tubers, is both a food source and a traditional Chinese medicinal plant. It offers significant nutritional and medicinal benefits.
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