AI Article Synopsis
- A 2000 base pair DNA fragment from the sea urchin histone gene has been isolated using the Eco R1 enzyme and cloned into a bacterial plasmid.
- This fragment includes sequences that correspond to two histone genes and contains specific cleavage sites for the enzymes Hind III and Hae III.
- Sequencing revealed 62 bp near the Hind III site and 42 bp near the Hae III site, confirming that the fragment codes for histone proteins H2A and H3, and providing their positions relative to the restriction sites.
Article Abstract
A 2000 base pair (bp) DNA fragment can be excised from sea urchin (S. purpuratus) histone gene repeat units with restriction endonuclease Eco R1. This DNA, which has been cloned in a bacterial plasmid, is known to encompass two of the five histone genes. The fragment has a single endonuclease Hind III cleavage site in one of the genes and a Hae III cleavage site in the other gene. We now report the nucleotide sequences of 62 bp adjacent to the Hind III site and 42 bp adjacent to the Hae III cleavage site. The results identify the cloned DNA as histone genes, show that it codes for histone proteins H2A and H3, and locate and orient H2A and H3 genes with respect to restriction endonuclease sites in the repeat unit.
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| http://dx.doi.org/10.1016/0092-8674(76)90031-3 | DOI Listing |
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