Radionuclide content in muscular and osseous tissues of elks and wild boars was determined by means of spectrum analysis. The radioactive caesium accumulation dynamics in muscular tissues of elks and wild boars from 1986 till 1992 years is presented.
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EMBO J
August 2024
Translational Cancer Medicine Research Program, University of Helsinki, Biomedicum Helsinki, Haartmaninkatu 8, 00290, Helsinki, Finland.
Migrating cells preferentially breach and integrate epithelial and endothelial monolayers at multicellular vertices. These sites are amenable to forces produced by the migrating cell and subsequent opening of the junctions. However, the cues that guide migrating cells to these entry portals, and eventually drive the transmigration process, are poorly understood.
View Article and Find Full Text PDFMurine models of obesity or reduced adiposity are a valuable resource for understanding the role of adipocyte dysfunction in metabolic disorders. Adipose tissue stromal vascular cells or primary adipocytes derived from murine adipose tissue and grown in culture are essential tools for studying the mechanisms underlying adipocyte development and function. Herein, we describe methods for the isolation, expansion, and long-term storage of murine adipose-derived stromal/stem cells, along with protocols for inducing adipogenesis to white or beige adipocytes in this cell population and osteogenic differentiation.
View Article and Find Full Text PDFJ Pediatr Hematol Oncol
March 2024
Pediatric Hematology/Oncology, Penn State College of Medicine, Hershey.
Anaplastic lymphoma kinase ( ALK )-fusion sarcomas are rare part of the emerging theoretically targetable tyrosine kinase RAS::MAPK pathway fusion myopericytic-ovoid sarcomas. We report our clinicopathologic and treatment experience with an ALK fusion sarcoma. A novel ELKS/RAB6-interacting/CAST family member 1 - unaligned ALK fusion infiltrative nonmetastatic low-grade sarcoma of the right hand of a 15-month-old male was treated with crizotinib, an ALK tyrosine kinase inhibitor as oral monotherapy, inducing complete radiographic and clinical resolution by 10 months and sustained response now over 12 months after elective discontinuation.
View Article and Find Full Text PDFJ Biol Chem
June 2023
Brain Research Center, Southern University of Science and Technology, Shenzhen, Guangdong, China; School of Life Sciences, Southern University of Science and Technology, Shenzhen, Guangdong, China. Electronic address:
ELKS proteins play a key role in organizing intracellular vesicle trafficking and targeting in both neurons and non-neuronal cells. While it is known that ELKS interacts with the vesicular traffic regulator, the Rab6 GTPase, the molecular basis governing ELKS-mediated trafficking of Rab6-coated vesicles, has remained unclear. In this study, we solved the Rab6B structure in complex with the Rab6-binding domain of ELKS1, revealing that a C-terminal segment of ELKS1 forms a helical hairpin to recognize Rab6B through a unique binding mode.
View Article and Find Full Text PDFInt J Mol Sci
April 2023
Department of Biochemistry, Faculty of Medicine, University of Yamanashi, Yamanashi 409-3898, Japan.
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