An expression vector designed to express a long (approximately 1 kb) RNA containing multiple ribozyme domains was cotransfected into mammalian cells with a plasmid encoding, as target, messenger RNA for chloramphenicol acetyltransferase (CAT). In comparative studies the multimeric ribozyme construct proved to be significantly more effective at suppressing CAT expression than either the corresponding antisense RNA, or a transcript carrying a single ribozyme domain. Suppression of gene activity was apparently specific because expression of an independently expressed gene for human growth hormone was unaffected. The profile of CAT RNA extracted from transfected cells was consistent with RNA cleavage.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1089/ard.1994.4.87 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Selective In Situ Analysis of Hepatogenic Exosomal microRNAs via Virus-Mimicking Multifunctional Magnetic Vesicles.
Adv Healthc Mater
January 2025
The Institute of Chinese Medicine of Nanjing University, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing University Drum Tower Hospital Clinical College, Nanjing University of Chinese Medicine, Nanjing, 210008, China.
Drug-induced liver injury (DILI) is a common clinical problem with urgent respect to demanding early diagnosis. Exosomal miRNAs are reliable and noninvasive biomarkers for the early diagnosis of DILI. However, accurate and feasible detection of exosomal miRNAs is often hampered by the low abundance of miRNAs, inefficient exosome separation techniques, and the requirement for RNA extraction from large sample volumes.
View Article and Find Full Text PDFEffects of the RNA-Binding Protein Sam68 on Poly(ADP-Ribose)polymerase 1 Activity.
Biochemistry (Mosc)
December 2024
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia.
Taking into account involvement of the RNA-binding proteins in regulation of activity of poly(ADP-ribose) polymerase 1 (PARP1), a key factor of DNA repair, the effect of the intrinsically disordered protein Sam68 (Src-associated substrate during mitosis of 68 kDa) on catalytic activity of this enzyme was studied. Plasmid containing coding sequence of the Sam68 protein was obtained. Using the obtained construct, conditions for the Sam68 expression in cells were optimized and procedure for protein purification was developed.
View Article and Find Full Text PDFMnGA with multiple enzyme-like properties for acute wound healing by reducing oxidative stress and modulating signaling pathways.
Mater Today Bio
February 2025
Research Center of Nanomedicine Technology, The Second Affiliated Hospital of Guangxi Medical University, Nanning, 530000, PR China.
Nanozymes with specific catalytic activity inhibit inflammation and promote wound healing efficiently and safely. In this work, multifunctional manganese-based nanozymes (MnGA) with antioxidant properties were successfully constructed via a simple coordination reaction in which manganese chloride was used as the manganese source and gallic acid (GA) was used as the ligand solution. MnGA possesses both catalase-like (CAT-like) and superoxide dismutase-like (SOD-like) activities and a reactive nitrogen species (RNS) scavenging capacity, which enables it to efficiently inhibit the inflammatory response.
View Article and Find Full Text PDFGroup V Chitin Deacetylases Are Responsible for the Structure and Barrier Function of the Gut Peritrophic Matrix in the Chinese Oak Silkworm .
Int J Mol Sci
December 2024
Liaoning Engineering and Technology Research Center for Insect Resources, College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China.
Chitin deacetylases (CDAs) are carbohydrate esterases associated with chitin metabolism and the conversion of chitin into chitosan. Studies have demonstrated that chitin deacetylation is essential for chitin organization and compactness and therefore influences the mechanical and permeability properties of chitinous structures, such as the peritrophic membrane (PM) and cuticle. In the present study, two genes ( and ) encoding CDA protein isoforms were identified and characterized in Chinese oak silkworm () larvae.
View Article and Find Full Text PDFImmunomodulatory Effects of SPHK1 and Its Interaction with TFAP2A in Yellow Drum ().
Int J Mol Sci
December 2024
State Key Laboratory of Mariculture Breeding, Key Laboratory of Healthy Mariculture for the East China Sea, Fisheries College, Jimei University, Xiamen 361021, China.
Sphingosine kinases (SPHKs) are essential enzymes that catalyze the phosphorylation of sphingosine to produce sphingosine-1-phosphate (S1P), which plays pivotal roles in inflammation and immune regulation. In this study, genome-wide association analysis (GWAS) identified the gene as closely associated with the resistance of yellow drum () to . Structural prediction showed that YDSPHK1 contains a typical diacylglycerol kinase catalytic (DAGKc) domain (154-291 aa).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!