From 237 children hospitalized in the enteric department of an infectious clinic 381 fecal samples were obtained and examined (after 24-hour pre-cultivation in selenite enrichment medium) in the latex agglutination test. The results thus obtained were compared with the data of the bacteriological study of these samples. For the first time the diagnostic value of the latex agglutination test (LAT) both for the early and retrospective diagnosis of Salmonella mono- and combined infections was established. The possibility of using LAT for augmenting the laboratory determination of salmonellosis, on the average, 2.1-fold in cases of acute enteric infections, including 3.3-fold in mild and non-manifest forms of such infections and 6.2-fold in clinical variants of the disease with lesions of the proximal sections of the gastrointestinal tract, was demonstrated.
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Antibiotics (Basel)
January 2025
Department of Oral Microbiology, Medical Faculty, Medical University of Gdansk, 80-204 Gdansk, Poland.
In the present study, we used phenotypic and molecular methods to determine susceptibility to oxacillin in coagulase-negative staphylococci (CoNS) and estimate the prevalence of strains with low-level resistance to oxacillin, A-positive oxacillin-susceptible methicillin-resistant (OS-MRCoNS), and borderline oxacillin-resistant (BORCoNS). One hundred one CoNS strains were screened for oxacillin and cefoxitin susceptibility using phenotypic (disk diffusion, agar dilution, latex agglutination, and chromagar) and molecular (detection of A, B, and C) methods. Staphylococcal cassette chromosome (SCC) typing was performed.
View Article and Find Full Text PDFBME Front
December 2024
Department of Laboratory Medicine and Center of Infectious Diseases and Pathogen Biology, The First Hospital of Jilin University, Changchun 130021, China.
This study aims to couple C-reactive protein (CRP) antibodies onto latex spheres of 2 different sizes to enhance the accuracy and sensitivity of CRP detection. Furthermore, it seeks to establish a robust methodological framework crucial for advancing the development of latex-enhanced immunoturbidimetric detection reagents. CRP, an acute-phase protein, rapidly elevates in response to infections or tissue damage.
View Article and Find Full Text PDFMicrobiol Immunol
December 2024
Department of Biology, West Chester University of Pennsylvania, West Chester, Pennsylvania, USA.
Antibiotic-resistant pathogens in public settings present a growing risk to human health. Staphylococcus aureus often asymptomatically colonizes human skin, while virulent strains cause soft tissue infections, osteomyelitis, endocarditis, and are associated with cystic fibrosis. Here we investigated the presence and distribution of multidrug-resistant S.
View Article and Find Full Text PDFAdv Lab Med
December 2024
Department of Laboratory Service, Área de Gestión Sanitaria Norte de Huelva, Hospital de Riotinto, Minas de Riotinto, Huelva, Spain.
Objectives: To describe a variant hemoglobin that interferes with HbA analysis by cation exchange HPLC.
Case Presentation: A 78 years-old Spanish male patient visited the Internal Medicine Clinic for a routine check-up, with HbA included to screen for diabetes. He had suffered hypertension and dyslipidemia, and the patient had no previous symptoms suggestive of diabetes such as hyperglycemia, weight loss, polydipsia, polyuria or tiredness.
Diagnostics (Basel)
November 2024
Department of Laboratory Medicine, Konkuk University School of Medicine, Seoul 05030, Republic of Korea.
The fecal calprotectin (f-Cal) test is a convenient method used for differentiating inflammatory bowel disease (IBD) from functional bowel disorders. The OC-Sensor Pledia (OC-FCa; Eiken Chemical Co., Tokyo, Japan) is a latex agglutination turbidimetric immunoassay used for f-Cal measurements.
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