The endo-polygalacturonase II from Aspergillus niger has been crystallized from an ammonium sulfate solution by the hanging drop method. The crystals belong to the monoclinic space group P2(1), with cell dimensions a = 69.6 A, b = 152.6 A, c = 74.0 A and beta = 91.2 degrees with four molecules per asymmetric unit. The crystals diffract to at least 2.8 A resolution and are suitable for X-ray analysis.
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http://dx.doi.org/10.1006/jmbi.1994.1660 | DOI Listing |
The food enzyme containing endo-polygalacturonase and β-glucosidase (EC 3.2.1.
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February 2025
Key Laboratory of Specialty Agri-product Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Sciences, China Jiliang University, Hangzhou 310018, China. Electronic address:
The endo-polygalacturonase D (PgaD) from Aspergillus niger JL15 was recombinantly expressed in Escherichia coli BL21, exhibiting an optimal activity at 55 °C and pH 4.0. Hydrolysis products of citrus pectin by recombinant PgaD included galacturonic acid (GalA), digalacturonic acid (GalA2), trigalacturonic acid (GalA3), and tetragalacturonic acid (GalA4).
View Article and Find Full Text PDFThe food enzyme has four declared activities: endo-polygalacturonase ((1-4)-α-d-galacturonan glycanohydrolase (endo-cleaving); EC 3.2.1.
View Article and Find Full Text PDFThe food enzyme endo-polygalacturonase ((1 → 4)--d-galacturonan glycanohydrolase EC 3.2.1.
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March 2024
University of Belgrade - Institute of Chemistry, Technology and Metallurgy - National Institute of the Republic of Serbia, Department of Chemistry, Belgrade, Republic of Serbia.
This study focuses on the isolation, purification, and characterisation of -polygalacturonase II from FAT43, particularly emphasising its potential applications in the fruit juice industry. A comprehensive screening test revealed the temporal dynamics of -polygalacturonase production during a 96-hour fermentation process. The purification process, involving ammonium sulfate and ethanol precipitation followed by ion-exchange chromatography, resulted in a 3.
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