The objectives of the present studies were to determine if the numbers of IGF-I receptors in bovine granulosa cells differed with size of follicle and to determine if growth factors and hormones affected the number of IGF-I receptors in granulosa cells. Granulosa cells from small (1-5 mm) and large (> or = 8 mm) follicles were cultured for 2-4 days in 10% FCS and then assessed for levels of IGF-I receptors. Numbers of IGF-I receptors were 15-fold greater in granulosa cells from large than small follicles. In addition, bFGF (5 and 50 ng/ml) decreased whereas estradiol (1 microgram/ml), FSH (10 ng/ml) and EGF (10 and 100 ng/ml) increased the number of IGF-I receptors in granulosa cells from small follicles. These hormones had no effect on the number of IGF-I receptors in granulosa cells from large follicles. In conclusion, granulosa cells from large follicles have a greater number of IGF-I receptors than cells from small follicles, and thus, it appears that granulosa cells acquire a greater number of IGF-I receptors during the process of differentiation.
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