Using the Tubercle bacillus Danish D1331 atoxic species as antigen and by single radial immunodiffusion (SRID) technic, the serum anti-TB-Ab of 454 patients with tuberculosis or other diseases were assayed and the results were reported. It was demonstrated that the sensitivity, specificity and accuracy of this method to diagnose tuberculosis were 81.78%, 97.92% and 90.31%; the positive and negative predictivities of this method were 97.22% and 85.77%, and the diagnostic efficiency was 80.08%. It was suggested this method had significant clinical value for diagnosing of tuberculosis.

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