AI Article Synopsis

  • IgE antibodies in people allergic to ant venom show reactivities to the venoms from various Myrmecia species (including M. pilosula and M. nigrocincta).
  • Most subjects showed reactivity primarily to M. pilosula venom, but all six Myrmecia venoms can induce these antibodies.
  • Analysis (SDS-PAGE and Western blotting) revealed multiple IgE-binding components in each venom, indicating that similar peptides may be present due to close genetic relationships among the species.

Article Abstract

IgE antibody reactivities to the venoms of Myrmecia pilosula, Myrmecia nigrocincta, Myrmecia tarsata, Myrmecia pyriformis, Myrmecia simillima and Myrmecia gulosa have been identified in sera from subjects allergic to ant venom. Sera with IgE reactivity to only a single Myrmecia venom most often recognize M. pilosula venom although all six venoms appear capable of inducing IgE antibodies. The six different Myrmecia venoms were electrophoretically separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western blotted to nitrocellulose and probed with sera from ant venom-allergic subjects. Four to six IgE-binding components ranging in size from 2 to 25 kDa were identified in each of the venoms. Similarities in molecular weights of the IgE-binding components exist and close taxonomic relationships between the species suggest that common or similar peptides may be present in the different venoms.

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Source
http://dx.doi.org/10.1111/j.1365-2222.1994.tb00957.xDOI Listing

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