The thymidylate synthase (TS) gene from Lactococcus lactis has been highly expressed in Escherichia coli. The TS protein was purified by sequential chromatography on Q-Sepharose and phenyl-Sepharose. Six grams of cell pellet yielded 140 mg of homogeneous TS. TS is a highly conserved enzyme, and several of the conserved amino acid residues that have been implicated in catalytic function are altered in L. lactis TS. By use of a 3-dimensional homology model, we have predicted covariant changes that might compensate for these differences. With the large amounts of L. lactis TS now available, studies can be pursued to understand the structure-function relationships of this enzyme compared to other TSs and to confirm the presumed roles of the compensatory changes predicted in the homology model.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2142890 | PMC |
| http://dx.doi.org/10.1002/pro.5560030715 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
TYMS Enhances Colorectal Cell Antioxidant Capacity Via the KEAP1-NRF2 Pathway to Resist Ferroptosis.
J Cancer
January 2025
The Colorectal and Anal Surgery Department of Shanxi Provincial People's Hospital, Shanxi Medical University, Taiyuan, China.
Article Synopsis
- Thymidylate synthase (TYMS) plays a crucial role in DNA synthesis and is found to be overexpressed in colorectal cancer (CRC) tissues, indicating a link to poor patient prognosis.
- TYMS overexpression enhances CRC cell proliferation and increases cellular antioxidant capacity by downregulating KEAP1 and promoting NRF2 translocation into the nucleus, which upregulates antioxidant genes.
- Inhibiting TYMS with 5-fluorouracil (5-FU) shows potential for drug synergism with erastin, suggesting a strategy to overcome TYMS-mediated resistance to ferroptosis in CRC treatment.
In silico bioprospecting of receptors for Oligoventin: An antimicrobial peptide isolated from spider eggs of Phoneutria nigriventer.
Colloids Surf B Biointerfaces
December 2024
Laboratory of Applied Toxicology, Center of Toxins, Immune-Response and Cell Signaling - CeT-ICS/CEPID, Butantan Institute São Paulo, São Paulo, SP CEP 05503-900, Brazil; Postgraduate Program Interunits in Biotechnology, USP/IPT/IBU, São Paulo, SP, Brazil. Electronic address:
Background: Irresponsible and wholesale use of antimicrobial agents is the principal cause of the emergence of strains of resistant microorganisms to traditional drugs. Oligoventin is a neutral peptide isolated from spider eggs of Phoneutria nigriventer, with antimicrobial activity against Gram-positive, Gram-negative, and yeast organisms. However, the molecular target and pathways of antimicrobial activity are still unknown.
View Article and Find Full Text PDFRational design, synthesis, biological evaluation, molecular docking, and molecular dynamics of substituted uracil derivatives as potent anti-cancer agents.
Bioorg Chem
December 2024
Department of Photochemistry (Synthetic Unit), Chemical Industries Research Institute, National Research Centre, Cairo 12622, Egypt. Electronic address:
An efficient synthesis of a series of uracil analogous was performed to obtain new potential anticancer agents. The cytotoxic effect of the synthesized derivatives was assessed in vitro against three cancer cell lines, namely hepatic cancer (HepG-2), colon cancer (HCT-116), and breast cancer (MCF-7). Among the tested compounds, 5, 11 and 15 stood as potent uracil derivatives with pan cytotoxicity against the 3 cell lines out-performing the reference compound 5-FU.
View Article and Find Full Text PDFDynamics, mechanistic and energetic evaluation of thiazole-thiadiazole compounds in flavin dependent thymidylate synthase of Mycobacterium tuberculosis.
Int J Biol Macromol
December 2024
Bioinformatics Centre, Savitribai Phule Pune University, Pune 411007, MS (Maharashtra), India. Electronic address:
Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), remains a significant global health challenge due to the emergence of drug-resistant strains. This study targets Flavin-dependent thymidylate synthase (ThyX), an essential enzyme in the thymidylate biosynthesis pathway crucial for bacterial DNA replication. We utilized advanced computational techniques, including molecular dynamics (MD) simulations and interaction energy analysis, to examine the binding interactions and stability of various thiazole-thiadiazole compounds with Mtb ThyX.
View Article and Find Full Text PDFEffect of Concentration of TiO Nanoparticles on Thiadiazole Derivative and Their Molecular Docking Study.
J Fluoresc
December 2024
Department of Studies in Physics, Karnatak University, Dharwad, 580003, Karnataka, India.
In the present work, we report the synthesis of TiO nanoparticles by hydrothermal method using titanium isopropoxide. The synthesized TiO nanoparticles were investigated by Powder X-ray diffraction, FE-SEM with EDX, Photoluminescence, UV-Visible absorption and Fluorescence emission spectroscopy. Fluorescence intensity and absorption values of 4-[5-(2,5-Dimethyl-pyrrol-1-yl)-[1,3,4]thiadiazol-2-ylsulfanylmethyl]-6-methoxy-chromen-2-one (DTYMC) molecule decreases with adding the concentration of TiO nanoparticles.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!