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Novel Decellularization Scheme for Preparing Acellular Fish Scale Scaffolds for Bone Tissue Engineering.
ACS Omega
January 2025
Department of Orthopedics, Peking University Third Hospital, No. 49 North Garden Road, Haidian, 100191 Beijing, China.
In bone tissue engineering, a suitable scaffold is the key. Due to their similar composition to bone tissue, special structure, good mechanical properties, and osteogenic properties, acellular fish scale scaffolds are potential scaffolds for bone tissue engineering. At present, the fish scale decellularization scheme mostly uses a combination of sodium dodecyl sulfate and ethylenediamine tetraacetic acid (EDTA), but this method has problems.
View Article and Find Full Text PDF[Establishment and Application of Efficient Gene Editing Method for Classical HLA-I Molecules].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
December 2024
Institute of Transfusion Medicine, Blood Center of Zhejiang Province, Hangzhou 310052, Zhejiang Province, China.
Objective: To establish an efficient gene editing method of HLA-I gene to prepare HLA-I universal hematopoietic stem cells.
Methods: The easyedit small guide RNA(sgRNA) was designed according to the sequences of β2 microglobulin gene and synthesized by GenScript company. RNP complexes were formed by NLS-Cas9-NLS nuclease and Easyedit sgRNA according to different molar ratios (1∶1~1∶4).
Photo-Cross-linked DNA Structures Greatly Improves Their Serum Nuclease Resistances and Gene Knock-In Efficiencies.
Small Methods
December 2024
Hefei National Research Center for Physical Sciences at the Microscale, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, 230026, China.
The stabilization and structural integrity of DNA architectures remain significant challenges in their biomedical applications, particularly when inserting functional units into the genome using long single-stranded DNA (lssDNA). To address these challenges, a site-specific photo-cross-linking method is employed. Single-stranded oligonucleotides, containing one or two photosensitive cyanovinylcarbazole nucleoside (K) molecules, are precisely incorporated and cross-linked at the specific sites of ssDNA through base-pairing, followed by rapid UV irradiation at 365 nm.
View Article and Find Full Text PDFUnveiling the Potential of Tetrahedral DNA Frameworks in Clinical Medicine: Mechanisms, Advances, and Future Perspectives.
Small
December 2024
State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Centre for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.
Article Synopsis
- DNA nanotechnology is making strides in treating clinical diseases, with tetrahedral frameworks nucleic acids (tFNAs) being a key innovation due to their easy design, cost-effectiveness, and high yield.
- tFNAs are resilient against immune responses and nuclease activity, and their structural programmability allows for the creation of functional and dynamic nanomaterials.
- The review highlights both the advantages of tFNAs—like biocompatibility and diverse applications in various medical fields—as well as the current limitations and challenges faced in research and clinical settings.
A minimal complex of KHNYN and zinc-finger antiviral protein binds and degrades single-stranded RNA.
Proc Natl Acad Sci U S A
December 2024
Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109.
Detecting viral infection is a key role of the innate immune system. The genomes of some RNA viruses have a high CpG dinucleotide content relative to most vertebrate cell RNAs, making CpGs a molecular marker of infection. The human zinc-finger antiviral protein (ZAP) recognizes CpG, mediates clearance of the foreign CpG-rich RNA, and causes attenuation of CpG-rich RNA viruses.
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