A simple method for identification of individuals and maternity testing has been developed. This technique includes PCR amplification of the 199-bp hypervariable portion of the noncoding region of human mtDNA, digestion with RsaI and subsequent SSCP analysis of restriction fragments with DNA silver staining. Using this approach we have analysed the DNA fingerprint patterns of the family members. The fingerprints of maternally related individuals appeared to be identical in three generations, while maternally unrelated members of the family showed differences in their fingerprints, either in SSCP or both RFLP and SSCP patterns. Sequencing data have confirmed the results obtained. Further DNA fingerprinting analysis of 19 unrelated mother-child pairs by means of the method described revealed complete identity of the fingerprint patterns within the pairs. The probability of a random fingerprint match for two maternally unrelated individuals was estimated as 8%.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/humu.1380030318 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Quantifying Bone Collagen Fingerprint Variation Between Species.
Mol Ecol Resour
January 2025
Manchester Institute of Biotechnology, School of Natural Sciences, University of Manchester, Manchester, UK.
Collagen is the most ubiquitous protein in the animal kingdom and one of the most abundant proteins on Earth. Despite having a relatively repetitive amino acid sequence motif that enables its triple helical structure, in type 1 collagen, that dominates skin and bone, there is enough variation for its increasing use for the biomolecular species identification of animal tissues processed or degraded beyond the amenability of DNA-based analyses. In recent years, this has been most commonly achieved through the technique of collagen peptide mass fingerprinting (PMF) known as ZooMS (Zooarchaeology by Mass Spectrometry), applied to the analysis of tens of thousands of samples across over one hundred studies in the past decade alone.
View Article and Find Full Text PDFSerum metabolomic signatures of patients with rare neurogenetic diseases: an insight into potential biomarkers and treatment targets.
Front Mol Neurosci
January 2025
Interdisciplinary Centre for Innovations in Biotechnology and Neuroscience, Faculty of Medical Sciences, University of Sri Jayewardenepura, Nugegoda, Sri Lanka.
Introduction: To further advance our understanding of Muscular Dystrophies (MDs) and Spinocerebellar Ataxias (SCAs), it is necessary to identify the biological patterns associated with disease pathology. Although progress has been made in the fields of genetics and transcriptomics, there is a need for proteomics and metabolomics studies. The present study aimed to be the first to document serum metabolic signatures of MDs (DMD, BMD, and LGMD 2A) SCAs (SCA 1-3), from a South Asian perspective.
View Article and Find Full Text PDFGenetically and chemically intraspecific variations of Ficus hirta provide novel insights into its protection and utilization.
BMC Plant Biol
January 2025
School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, 510515, China.
Background: Radix Fici Hirtae, the dry root of Ficus hirta, is a famous ethnomedicine and food that has been widely used by Yao and Zhuang nationalities in southern China for its potent antitumor, antifungal, and hepatoprotective effects. Recently, owing to over-exploitation and habitat destruction, F. hirta has been pushed to the brink of depletion.
View Article and Find Full Text PDFEnhancing trace DNA profile recovery in forensic casework using the amplicon RX post-PCR clean-up kit.
Sci Rep
January 2025
The Biology and DNA Section, General Department of Forensic Science and Criminology, Dubai Police General Head Quarters, Dubai, United Arab Emirates.
This study evaluated the effectiveness of the amplicon RX post-PCR clean-up kit in enhancing trace DNA profile recovery from forensic casework samples amplified using the GlobalFiler PCR amplification kit. The impact of post-PCR clean-up on allele recovery and signal intensity was assessed in both trace casework samples and control samples across a range of DNA concentrations. The results showed that the amplicon RX method significantly improved allele recovery compared to the 29-cycle protocol (p = 8.
View Article and Find Full Text PDFConcordant Patterns of Population Genetic Structure in Food-Deceptive Orchids.
Genes (Basel)
January 2025
Faculty of Biology, University of Bialystok, Ciołkowskiego 1J Street, 15-245 Białystok, Poland.
Background: The patterns of inbreeding coefficients () and fine spatial genetic structure (FSGS) were evaluated regarding the mating system and inbreeding depression of food-deceptive orchids, , var. , and , from NE Poland.
Methods: We used 455 individuals, representing nine populations of three taxa and AFLPs, to estimate percent polymorphic loci and Nei's gene diversity, which are calculated using the Bayesian method; ; ; FSGS with the pairwise kinship coefficient (); and AMOVA in populations.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!